This review compiles research results published over the last 14 years on conifer somatic
embryogenesis (SE). Emphasis is placed on the newest findings that affect the response of …

Somatic embryogenesis was achieved in the conifers Pinus nigra Arn. and in the hybrids
Abies alba× A. cephalonica and Abies alba× A. numidica. For initiation of embryogenic …

In the face of habitat loss and a changing climate, there is a growing need for increased
efforts to conserve plant biodiversity. The genetic diversity of many crops is well represented …

Atlantic white cedar (AWC; Chamaecyparis thyoides), an aromatic evergreen conifer native
to swamps and bogs along the Atlantic and Gulf coasts of the eastern United States was …

The development of a vitrification method for cryopreservation of embryogenic lines from
mature holm oak (Quercus ilex L.) trees is reported. Globular embryogenic clusters of three …

Initiation of somatic embryogenesis from immature zygotic embryos, long-term maintenance
of embryogenic tissue in vitro or by cryopreservation, as well as maturation, of somatic …

Embryogenic tissues of Abies alba Mill. were cryopreserved using the slow-freezing
approach. Four cell lines were incubated for 24 h on a medium with 0.5 M sorbitol and pre …

Cryopreservation, or the storage at liquid nitrogen temperatures (-196° C), of embryogenic
cells or somatic embryos allows their long-term conservation without loss of their …

The combined approaches between ex situ and in situ conservation are of great importance
for threatened species in urgent need of protection. This study aims to develop concrete …

The conservation of threatened exceptional plants, which cannot be conserved by seed
banking, requires in vitro technologies for many of the approaches needed for their long …