Organoids are promising tools for species‐specific in vitro toxicological studies

J Augustyniak, A Bertero, T Coccini… - Journal of Applied …, 2019 - Wiley Online Library
Organoids are three‐dimensional self‐aggregating structures generated from stem cells
(SCs) or progenitor cells in a process that recapitulates molecular and cellular stages of …

Pluripotent stem cells from domesticated mammals

T Ezashi, Y Yuan, RM Roberts - Annual review of animal …, 2016 - annualreviews.org
This review deals with the latest advances in the study of embryonic stem cells (ESC) and
induced pluripotent stem cells (iPSC) from domesticated species, with a focus on pigs, cattle …

Effect of antioxidants and apoptosis inhibitors on cryopreservation of murine germ cells enriched for spermatogonial stem cells

SJ Ha, BG Kim, YA Lee, YH Kim, BJ Kim, SE Jung… - PLoS …, 2016 - journals.plos.org
Spermatogonial stem cells (SSCs) are germline stem cells that serve as the foundation of
spermatogenesis to maintain fertility throughout a male's lifetime. To treat male infertility …

In vitro breeding: application of embryonic stem cells to animal production

DE Goszczynski, H Cheng… - Biology of …, 2019 - academic.oup.com
Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation
blastocysts. For decades, attempts to efficiently derive ESCs in animal livestock species …

Establishment of porcine embryonic stem cells in simplified serum free media and feeder free expansion

H Choi, D Oh, M Kim, A Jawad, H Zheng, L Cai… - Stem Cell Research & …, 2024 - Springer
Background The establishment of stable porcine embryonic stem cells (pESCs) can
contribute to basic and biomedical research, including comparative developmental biology …

Livestock pluripotency is finally captured in vitro

M Navarro, DA Soto, CA Pinzon, J Wu… - Reproduction, Fertility …, 2020 - CSIRO Publishing
Pluripotent stem cells (PSCs) have demonstrated great utility in improving our
understanding of mammalian development and continue to revolutionise regenerative …

An optimized culture system for efficient derivation of porcine expanded potential stem cells from preimplantation embryos and by reprogramming somatic cells

D Ruan, Y Xuan, TTKK Tam, ZX Li, X Wang, S Xu… - Nature …, 2024 - nature.com
Pigs share anatomical and physiological traits with humans and can serve as a large-animal
model for translational medicine. Bona fide porcine pluripotent stem cells (PSCs) could …

Blastomere aggregation using phytohemagglutinin-L improves the establishment efficiency of porcine parthenogenesis-derived embryonic stem-like cell lines

J Lee, L Cai, M Kim, H Choi, D Oh, A Jawad… - Frontiers in Cell and …, 2022 - frontiersin.org
Aggregation of blastomeres is a promising method to improve the developmental
competence of blastocysts and may be useful for the production of chimeric animals and the …

Lineage specification revealed by single-cell gene expression analysis in porcine preimplantation embryos

Q Wei, R Li, L Zhong, H Mu, S Zhang… - Biology of …, 2018 - academic.oup.com
After zygotic genome activation and lineage specification, zygotes develop into late
blastocysts comprising three distinct cell types. The molecular mechanisms underlying this …

A Rho-associated coiled-coil containing kinase inhibitor, Y-27632, improves viability of dissociated single cells, efficiency of colony formation, and cryopreservation in …

SK Baek, YS Cho, IS Kim, SB Jeon, DK Moon… - Cellular …, 2019 - liebertpub.com
The establishment of porcine epiblast stem cells (pEpiSCs) and induced pluripotent stem
cells (piPSCs) derived from diametrical derivations is of great importance in developing …