Novel non-nucleic acid targets detection strategies based on CRISPR/Cas toolboxes: A review

X Cheng, Y Li, J Kou, D Liao, W Zhang, L Yin… - Biosensors and …, 2022 - Elsevier
Various non-nucleic acid targets (ions, small molecules, polysaccharides, peptides/proteins/
enzymes, cells, transcription factors) are important biomarkers. They play important roles in …

State of the art in CRISPR/Cas system-based signal conversion and amplification applied in the field of food analysis

B Lu, Z Guo, K Zhong, T Osire, Y Sun, L Jiang - Trends in Food Science & …, 2023 - Elsevier
Background Food safety is a major global public health issue. The majority of traditional
culture-based methods have lengthy detection times, require sophisticated sample …

SERS-based CRISPR/Cas assay on microfluidic paper analytical devices for supersensitive detection of pathogenic bacteria in foods

J Zhuang, Z Zhao, K Lian, L Yin, J Wang, S Man… - Biosensors and …, 2022 - Elsevier
Rapid and point-of-need (PON) detection of bacteria is crucial to directly provide rapid and
reliable diagnostics information during on-site tests, allowing more room for taking proactive …

CRISPR‐Cas‐based detection for food safety problems: Current status, challenges, and opportunities

Y Li, S Man, S Ye, G Liu, L Ma - Comprehensive Reviews in …, 2022 - Wiley Online Library
Food safety is one of the biggest public issues occurring around the world. Microbiological,
chemical, and physical hazards can lead to food safety issues, which may occur at all stages …

A SERS-signalled, CRISPR/Cas-powered bioassay for amplification-free and anti-interference detection of SARS-CoV-2 in foods and environmental samples using a …

L Ma, W Zhang, L Yin, Y Li, J Zhuang, L Shen… - Journal of hazardous …, 2023 - Elsevier
The pandemic of COVID-19 creates an imperative need for sensitive and portable detection
of SARS-CoV-2. We devised a SERS-read, CRISPR/Cas-powered nanobioassay, termed as …

CRISPR‐Cas biochemistry and CRISPR‐based molecular diagnostics

Z Weng, Z You, J Yang, N Mohammad… - Angewandte Chemie …, 2023 - Wiley Online Library
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in
disease diagnostics, pathogen surveillance, and many more. However, this method requires …

CRISPR/Cas12a-based technology: A powerful tool for biosensing in food safety

Z Mao, R Chen, X Wang, Z Zhou, Y Peng, S Li… - Trends in Food Science …, 2022 - Elsevier
Background In the context of the current pandemic caused by the novel coronavirus,
molecular detection is not limited to the clinical laboratory, but also faces the challenge of …

Argonaute-triggered visual and rebuilding-free foodborne pathogenic bacteria detection

Y Li, J Kou, X Han, J Qiao, W Zhang, S Man… - Journal of Hazardous …, 2023 - Elsevier
Foodborne pathogenic bacteria are recognized as the main causes of microbial
contamination in food safety. Early screening and ultrasensitive detection of foodborne …

A ratiometric fluorescent biosensing platform for ultrasensitive detection of Salmonella typhimurium via CRISPR/Cas12a and silver nanoclusters

L Ma, J Wang, Y Li, D Liao, W Zhang, X Han… - Journal of Hazardous …, 2023 - Elsevier
Highlights•We develop a versatile biosensing platform for ultrasensitive detection of
pathogenic bacteria, termed as SCENT-Cas.•SCENT-Cas adopts ratiometric fluorescence …

[HTML][HTML] Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections

Y Wu, D Battalapalli, MJ Hakeem, V Selamneni… - Journal of …, 2021 - Springer
Antibiotic resistance is spreading rapidly around the world and seriously impeding efforts to
control microbial infections. Although nucleic acid testing is widely deployed for the …