Exploitation of Bacillus subtilis as a robust workhorse for production of heterologous proteins and beyond

W Cui, L Han, F Suo, Z Liu, L Zhou, Z Zhou - World Journal of Microbiology …, 2018 - Springer
Bacillus subtilis, belonging to the type species of Bacillus, is a type of soil-derived, low% G+
C, endospore-forming Gram-positive bacterium. After the discovery of B. subtilis 168 that …

An overview and future prospects of recombinant protein production in Bacillus subtilis

H Yang, J Qu, W Zou, W Shen, X Chen - Applied Microbiology and …, 2021 - Springer
Bacillus subtilis is a well-characterized Gram-positive bacterium and a valuable host for
recombinant protein production because of its efficient secretion ability, high yield, and non …

Microbial induced calcium carbonate precipitation study using Bacillus subtilis with application to self-healing concrete preparation and characterization

J Feng, B Chen, W Sun, Y Wang - Construction and Building Materials, 2021 - Elsevier
This study aimed to develop novel bacteria-based self-healing concrete by microbial
induced calcium carbonate precipitation mechanism. A group of spore-forming bacterium …

The multifunctionality of expression systems in Bacillus subtilis: Emerging devices for the production of recombinant proteins

CC Souza, JM Guimarães, SS Pereira… - Experimental …, 2021 - journals.sagepub.com
Bacillus subtilis is a successful host for producing recombinant proteins. Its GRAS (generally
recognized as safe) status and its remarkable innate ability to absorb and incorporate …

Promoter engineering enables overproduction of foreign proteins from a single copy expression cassette in Bacillus subtilis

C Zhou, B Ye, S Cheng, L Zhao, Y Liu, J Jiang… - Microbial cell …, 2019 - Springer
Background Bacillus subtilis is developed to be an attractive expression host to produce
both secreted and cytoplasmic proteins owing to its prominent biological characteristics …

Important role of Bacillus subtilis as a probiotic and vaccine carrier in animal health maintenance

C Yuan, X Ji, Y Zhang, X Liu, L Ding, J Li, S Ren… - World Journal of …, 2024 - Springer
Bacillus subtilis is a widespread Gram-positive facultative aerobic bacterium that is
recognized as generally safe. It has shown significant application value and great …

Scalable cultivation of engineered cyanobacteria for squalene production from industrial flue gas in a closed photobioreactor

SY Choi, SJ Sim, SC Ko, J Son, JS Lee… - Journal of agricultural …, 2020 - ACS Publications
Economically feasible photosynthetic cultivation of microalgal and cyanobacterial strains is
crucial for the biological conversion of CO2 and potential CO2 mitigation to challenge global …

Construction and application of a dual promoter system for efficient protein production and metabolic pathway enhancement in Bacillus licheniformis

Y Rao, D Cai, H Wang, Y Xu, S Xiong, L Gao… - Journal of …, 2020 - Elsevier
Promoter plays the critical role in regulating gene transcription, and dual-promoter has
received the widespread attentions due to its high efficiency and continuity, here, we want to …

Enhancement of edeine production in Brevibacillus brevis X23 via in situ promoter engineering

Q Liu, L Zhang, Y Wang, C Zhang, T Liu… - Microbial …, 2022 - Wiley Online Library
Edeines, a group of cationic antimicrobial peptides produced by the soil bacterium
Brevibacillus, have broad biological effects, such as antimicrobial, anticancer and …

[HTML][HTML] Integrative expression vectors with Pgrac promoters for inducer-free overproduction of recombinant proteins in Bacillus subtilis

DTM Tran, TTP Phan, TTN Doan, TL Tran… - Biotechnology …, 2020 - Elsevier
Inducer-free integrative vectors are often used to create B. subtilis strains for industrial
purposes, but employing strong promoters to produce high levels of recombinant proteins in …