Pyrosequencing: Current forensic methodology and future applications—A review

M Ghemrawi, NF Tejero, G Duncan… - Electrophoresis, 2023 - Wiley Online Library
The recent development of small, single‐amplicon‐based benchtop systems for
pyrosequencing has opened up a host of novel procedures for applications in forensic …

Challenges and (un) certainties for DNAm age estimation in future

H Correia Dias, E Cunha, F Corte Real, L Manco - Forensic Sciences, 2022 - mdpi.com
Age estimation is a paramount issue in criminal, anthropological, and forensic research.
Because of this, several areas of research have focused on the establishment of new …

Evidence for differences in DNA methylation between Germans and Japanese

J Becker, P Böhme, A Reckert, SB Eickhoff… - International Journal of …, 2022 - Springer
As a contribution to the discussion about the possible effects of ethnicity/ancestry on age
estimation based on DNA methylation (DNAm) patterns, we directly compared age …

[HTML][HTML] Predicting age from blood by droplet digital PCR using a set of three DNA methylation markers

HC Dias, L Manco - Forensic Science International, 2024 - Elsevier
Abstract Evaluation of DNA methylation (DNAm) patterns is a promising tool for age
estimation. The duplex droplet digital PCR (ddPCR) method has been recently investigated …

Altered DNA methylation at age-associated CpG sites in children with growth disorders: impact on age estimation?

F Mayer, J Becker, C Reinauer, P Böhme… - International journal of …, 2022 - Springer
Age estimation based on DNA methylation (DNAm) can be applied to children, adolescents
and adults, but many CG dinucleotides (CpGs) exhibit different kinetics of age-associated …

A semen‐specific deoxyribonucleic acid methylation model for epigenetic age estimation and its robustness under environmental challenges

S Er, Z Abik, G Ersoy, G Filoglu, H Ozkara… - …, 2024 - Wiley Online Library
In forensic investigations, semen samples are a common form of biological evidence,
especially in cases involving sexual assault. Therefore, accurately estimating the age of an …

[HTML][HTML] ELOVL2, PRKG2, and EDARADD DNA Methylation Strongly Estimate Indonesian Adolescents

N Soedarsono, MGS Hanafi, BT Hartomo, EI Auerkari - Diagnostics, 2024 - mdpi.com
Recently, there has been a growing interest in using DNA methylation analysis for age
estimation. Despite this growing interest, there is a scarcity of research on the potential of …

Detection of outlier methylation from bisulfite sequencing data with novel Bioconductor package BOREALIS

GR Oliver, G Jenkinson, RJ Olson, LE Schultz-Rogers… - bioRxiv, 2022 - biorxiv.org
DNA sequencing results in genetic diagnosis of 18-40% of previously unsolved cases, while
the incorporation of RNA-Seq analysis has more recently been shown to generate …