New twists in detecting mRNA modification dynamics
Modified nucleotides in mRNA are an essential addition to the standard genetic code of four
nucleotides in animals, plants, and their viruses. The emerging field of epitranscriptomics …
nucleotides in animals, plants, and their viruses. The emerging field of epitranscriptomics …
Naturally occurring modified ribonucleosides
PJ McCown, A Ruszkowska, CN Kunkler… - Wiley …, 2020 - Wiley Online Library
The chemical identity of RNA molecules beyond the four standard ribonucleosides has
fascinated scientists since pseudouridine was characterized as the “fifth” ribonucleotide in …
fascinated scientists since pseudouridine was characterized as the “fifth” ribonucleotide in …
Dynamic RNA acetylation revealed by quantitative cross-evolutionary mapping
Abstract N 4-acetylcytidine (ac4C) is an ancient and highly conserved RNA modification that
is present on tRNA and rRNA and has recently been investigated in eukaryotic mRNA …
is present on tRNA and rRNA and has recently been investigated in eukaryotic mRNA …
The structural and functional workings of KEOPS
J Beenstock, F Sicheri - Nucleic Acids Research, 2021 - academic.oup.com
Abstract KEOPS (K inase, E ndopeptidase and O ther P roteins of S mall size) is a five-
subunit protein complex that is highly conserved in eukaryotes and archaea and is essential …
subunit protein complex that is highly conserved in eukaryotes and archaea and is essential …
Pytheas: a software package for the automated analysis of RNA sequences and modifications via tandem mass spectrometry
L D'Ascenzo, AM Popova, S Abernathy… - Nature …, 2022 - nature.com
Mass spectrometry is an important method for analysis of modified nucleosides ubiquitously
present in cellular RNAs, in particular for ribosomal and transfer RNAs that play crucial roles …
present in cellular RNAs, in particular for ribosomal and transfer RNAs that play crucial roles …
Stress-induced modification of Escherichia coli tRNA generates 5-methylcytidine in the variable loop
S Valesyan, M Jora, B Addepalli… - Proceedings of the …, 2024 - pnas.org
There has been recent interest in trying to understand the connection between transfer RNA
(tRNA) posttranscriptional modifications and changes in-cellular environmental conditions …
(tRNA) posttranscriptional modifications and changes in-cellular environmental conditions …
Chemical amination/imination of carbonothiolated nucleosides during RNA hydrolysis
Liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) has become the gold‐
standard technique to study RNA and its various modifications. While most research on RNA …
standard technique to study RNA and its various modifications. While most research on RNA …
Instrumental analysis of RNA modifications
Y Yoluç, G Ammann, P Barraud, M Jora… - Critical Reviews in …, 2021 - Taylor & Francis
Organisms from all domains of life invest a substantial amount of energy for the introduction
of RNA modifications into nearly all transcripts studied to date. Instrumental analysis of RNA …
of RNA modifications into nearly all transcripts studied to date. Instrumental analysis of RNA …
Higher-energy collisional dissociation mass spectral networks for the rapid, semi-automated characterization of known and unknown ribonucleoside modifications
M Jora, D Corcoran, GG Parungao, PA Lobue… - Analytical …, 2022 - ACS Publications
Higher-energy collisional dissociation (HCD) of modified ribonucleosides generates
characteristic and highly reproducible nucleoside-specific tandem mass spectra (MS/MS) …
characteristic and highly reproducible nucleoside-specific tandem mass spectra (MS/MS) …
Commonality and diversity in tRNA substrate recognition in t6A biogenesis by eukaryotic KEOPSs
Abstract N 6-Threonylcarbamoyladenosine (t6A) is a universal and pivotal tRNA
modification. KEOPS in eukaryotes participates in its biogenesis, whose mutations are …
modification. KEOPS in eukaryotes participates in its biogenesis, whose mutations are …