A decade of advances in transposon-insertion sequencing
It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …
methods, which combine genome-wide transposon mutagenesis with high-throughput …
Transposon insertion sequencing, a global measure of gene function
T van Opijnen, HL Levin - Annual review of genetics, 2020 - annualreviews.org
The goal of genomics and systems biology is to understand how complex systems of factors
assemble into pathways and structures that combine to form living organisms. Great …
assemble into pathways and structures that combine to form living organisms. Great …
Role of the CrcB transporter of Pseudomonas putida in the multi‐level stress response elicited by mineral fluoride
The presence of mineral fluoride (F−) in the environment has both a geogenic and
anthropogenic origin, and the halide has been described to be toxic in virtually all living …
anthropogenic origin, and the halide has been described to be toxic in virtually all living …
Distinct bacterial pathways influence the efficacy of antibiotics against Mycobacterium tuberculosis
Effective tuberculosis treatment requires at least 6 months of combination therapy.
Alterations in the physiological state of the bacterium during infection are thought to reduce …
Alterations in the physiological state of the bacterium during infection are thought to reduce …
Air pollution and age-dependent changes in emotional behavior across early adolescence in the US
CE Campbell, DL Cotter, KL Bottenhorn, E Burnor… - Environmental …, 2024 - Elsevier
Recent studies have linked air pollution to increased risk for behavioral problems during
development, albeit with inconsistent findings. Additional longitudinal studies are needed …
development, albeit with inconsistent findings. Additional longitudinal studies are needed …
Oxidative damage and delayed replication allow viable Mycobacterium tuberculosis to go undetected
K Saito, S Mishra, T Warrier, N Cicchetti, J Mi… - Science translational …, 2021 - science.org
“Viable but nonculturable” states of bacteria pose challenges for environmental and clinical
microbiology, but their biological mechanisms remain obscure. Mycobacterium tuberculosis …
microbiology, but their biological mechanisms remain obscure. Mycobacterium tuberculosis …
Pathogen clonal expansion underlies multiorgan dissemination and organ-specific outcomes during murine systemic infection
K Hullahalli, MK Waldor - Elife, 2021 - elifesciences.org
The dissemination of pathogens through blood and their establishment within organs lead to
severe clinical outcomes. However, the within-host dynamics that underlie pathogen spread …
severe clinical outcomes. However, the within-host dynamics that underlie pathogen spread …
The Increased Accumulation of Staphylococcus aureus Virulence Factors Is Maximized in a purR Mutant by the Increased Production of SarA and Decreased …
Mutation of purR was previously shown to enhance the virulence of Staphylococcus aureus
in a murine sepsis model, and this cannot be fully explained by increased expression of …
in a murine sepsis model, and this cannot be fully explained by increased expression of …
[PDF][PDF] Comprehensive understanding of Tn5 insertion preference improves transcription regulatory element identification
Tn5 transposase, which can efficiently tagment the genome, has been widely adopted as a
molecular tool in next-generation sequencing, from short-read sequencing to more complex …
molecular tool in next-generation sequencing, from short-read sequencing to more complex …
Pangenome evaluation of gene essentiality in Streptococcus pyogenes
Bacterial species often consist of strains with variable gene content, collectively referred to
as the pangenome. Variations in the genetic makeup of strains can alter bacterial physiology …
as the pangenome. Variations in the genetic makeup of strains can alter bacterial physiology …