l-Isoleucine dioxygenase (IDO) directly catalyzes the C–H bond hydroxylation of several
hydrophobic aliphatic amino acids. However, the ambiguous selectivity of IDO prevents its …

Tungsten-dependent aldehyde oxidoreductases (AORs) catalyze the oxidation of aldehydes
to acids and are the only known enzymes reducing non-activated acids using electron …

In contrast to their molybdenum dependent relatives, tungsten enzymes operate at
significantly lower redox potentials, and in some cases they can carry out reversible redox …

The reaction of benzylsuccinate synthase, the radical-based addition of toluene to a
fumarate cosubstrate, is initiated by hydrogen transfer from a conserved cysteine to the …

Anaerobic toluene degradation proceeds by fumarate addition to produce (R)‐
benzylsuccinate as first intermediate, which is further degraded via β‐oxidation by five …

(R)-Benzylsuccinate is generated in anaerobic toluene degradation by the radical addition
of toluene to fumarate and further degraded to benzoyl-CoA by a β-oxidation pathway. Using …

Nitrilase can catalyze nitrile compounds to generate corresponding carboxylic acids.
Nitrilases as promiscuous enzymes can catalyze a variety of nitrile substrates, such as …

Anaerobic toluene degradation involves β‐oxidation of the first intermediate (R)‐2‐
benzylsuccinate to succinyl‐CoA and benzoyl‐CoA. Here, we characterize the last enzyme …

We characterise a reversible bacterial zinc-containing benzyl alcohol dehydrogenase
(BaDH) accepting either NAD+ or NADP+ as a redox cofactor. Remarkably, its redox …

Anaerobic microbial degradation of hydrocarbons is often initiated through addition of the
hydrocarbon to fumarate by enzymes known as X-succinate synthases (XSSs). XSSs use a …