High-throughput glycomic methods

I Trbojević-Akmačić… - Chemical …, 2022 - ACS Publications
Glycomics aims to identify the structure and function of the glycome, the complete set of
oligosaccharides (glycans), produced in a given cell or organism, as well as to identify …

Glycoproteomic analysis of antibodies

G Zauner, MHJ Selman, A Bondt, Y Rombouts… - Molecular & Cellular …, 2013 - ASBMB
Antibody glycosylation has been shown to change with various processes. This review
presents mass spectrometric approaches for antibody glycosylation analysis at the level of …

[PDF][PDF] Wang

Y Wang - Jia Z, Yi Q, Song L. The various components implied …, 2018 - tranassoc.com
Systems and methods are disclosed for communicating data in an integrated sensor network
having one or more nodes coupled to an optical sensor network and a radio frequency …

Linkage-Specific in Situ Sialic Acid Derivatization for N-Glycan Mass Spectrometry Imaging of Formalin-Fixed Paraffin-Embedded Tissues

S Holst, B Heijs, N De Haan, RJM Van Zeijl… - Analytical …, 2016 - ACS Publications
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging is a rapidly
evolving field in which mass spectrometry techniques are applied directly on tissues to …

High-throughput IgG Fc N-glycosylation profiling by mass spectrometry of glycopeptides

MP Baković, MHJ Selman, M Hoffmann… - Journal of proteome …, 2013 - ACS Publications
Age and sex dependence of subclass specific immunoglobulin G (IgG) Fc N-glycosylation
was evaluated for 1709 individuals from two isolated human populations. IgGs were …

Comparative performance of four methods for high-throughput glycosylation analysis of immunoglobulin G in genetic and epidemiological research

JE Huffman, M Pučić-Baković, L Klarić, R Hennig… - Molecular & Cellular …, 2014 - ASBMB
The biological and clinical relevance of glycosylation is becoming increasingly recognized,
leading to a growing interest in large-scale clinical and population-based studies. In the past …

Quantitative mapping of glycoprotein micro‐heterogeneity and macro‐heterogeneity: an evaluation of mass spectrometry signal strengths using synthetic peptides and …

K Stavenhagen, H Hinneburg… - Journal of Mass …, 2013 - Wiley Online Library
Mass spectrometry (MS) is used to quantify the relative distribution of glycans attached to
particular protein glycosylation sites (micro‐heterogeneity) and evaluate the molar site …

Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles—part 2: mass spectrometric methods

D Reusch, M Haberger, D Falck, B Peter, B Maier… - MAbs, 2015 - Taylor & Francis
To monitor the Fc glycosylation of therapeutic immunoglobulin G in bioprocess
development, product characterization and release analytics, reliable techniques for …

Advances in mass spectrometry‐based glycoproteomics

A Yu, J Zhao, W Peng, A Banazadeh… - …, 2018 - Wiley Online Library
Protein glycosylation, an important PTM, plays an essential role in a wide range of biological
processes such as immune response, intercellular signaling, inflammation, and host …

2, 5-Dihydroxyterephthalic Acid: A Matrix for Improved Detection and Imaging of Amino Acids

J Fu, J Gu, Z Bao, Y Zhou, H Hu, C Yang… - Analytical …, 2023 - ACS Publications
Amino acids (AAs), which are low-molecular-weight (low-MW) metabolites, serve as
essential building blocks not only for protein synthesis but also for maintaining the nitrogen …