Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

This review highlights recent efforts on applying electron microscopy (EM) to soft (including
biological) nanomaterials. We will show how developments of both the hardware and …

Cryo-focused ion beam milling of frozen-hydrated cells and subsequent cryo-electron
tomography (cryo-ET) has enabled the structural elucidation of macromolecular complexes …

INTRODUCTION Our textbook understanding of the nanoscale organization of the cell and
its relationship to the thousands of proteins that drive cellular metabolism comes largely from …

Cryogenic electron microscopy (cryo-EM) can reveal the molecular details of biological
processes in their native, cellular environment at atomic resolution. However, few cells are …

Cryofixation has proven to be the gold standard for efficient preservation of native cell
ultrastructure compared to chemical fixation, but this approach is not widely used in …

Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB)
has matured into a preparation method widely used for cellular cryo-electron tomography …

Chloroplast function is orchestrated by the organelle's intricate architecture. By combining
cryo-focused ion beam milling of vitreous Chlamydomonas cells with cryo-electron …

Electron microscopy played a key role in establishing cell biology as a discipline, by
producing fundamental insights into cellular organization and ultrastructure. Many seminal …

Cryoelectron tomography provides unprecedented insights into the macromolecular and
supramolecular organization of cells in a close-to-living state. However because of the …