Recent progress in mass spectrometry-based strategies for elucidating protein–protein interactions
Protein–protein interactions are fundamental to various aspects of cell biology with many
protein complexes participating in numerous fundamental biological processes such as …
protein complexes participating in numerous fundamental biological processes such as …
[HTML][HTML] Proteomic discovery of chemical probes that perturb protein complexes in human cells
Most human proteins lack chemical probes, and several large-scale and generalizable small-
molecule binding assays have been introduced to address this problem. How compounds …
molecule binding assays have been introduced to address this problem. How compounds …
Microglial-derived C1q integrates into neuronal ribonucleoprotein complexes and impacts protein homeostasis in the aging brain
Neuroimmune interactions mediate intercellular communication and underlie critical brain
functions. Microglia, CNS-resident macrophages, modulate the brain through direct physical …
functions. Microglia, CNS-resident macrophages, modulate the brain through direct physical …
Translational remodeling by RNA‐binding proteins and noncoding RNAs
Responsible for generating the proteome that controls phenotype, translation is the ultimate
convergence point for myriad upstream signals that influence gene expression. System …
convergence point for myriad upstream signals that influence gene expression. System …
Denaturing purifications demonstrate that PRC2 and other widely reported chromatin proteins do not appear to bind directly to RNA in vivo
Summary Polycomb repressive complex 2 (PRC2) is reported to bind to many RNAs and
has become a central player in reports of how long non-coding RNAs (lncRNAs) regulate …
has become a central player in reports of how long non-coding RNAs (lncRNAs) regulate …
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous
macromolecular networks on a proteome scale, but current methods are experimentally …
macromolecular networks on a proteome scale, but current methods are experimentally …
hu. MAP 2.0: integration of over 15,000 proteomic experiments builds a global compendium of human multiprotein assemblies
A general principle of biology is the self‐assembly of proteins into functional complexes.
Characterizing their composition is, therefore, required for our understanding of cellular …
Characterizing their composition is, therefore, required for our understanding of cellular …
The Sox2 transcription factor binds RNA
Certain transcription factors are proposed to form functional interactions with RNA to
facilitate proper regulation of gene expression. Sox2, a transcription factor critical for …
facilitate proper regulation of gene expression. Sox2, a transcription factor critical for …
Meta-analysis defines principles for the design and analysis of co-fractionation mass spectrometry experiments
MA Skinnider, LJ Foster - Nature Methods, 2021 - nature.com
Co-fractionation mass spectrometry (CF-MS) has emerged as a powerful technique for
interactome mapping. However, there is little consensus on optimal strategies for the design …
interactome mapping. However, there is little consensus on optimal strategies for the design …
RBP2GO: a comprehensive pan-species database on RNA-binding proteins, their interactions and functions
M Caudron-Herger, RE Jansen, E Wassmer… - Nucleic acids …, 2021 - academic.oup.com
RNA–protein complexes have emerged as central players in numerous key cellular
processes with significant relevance in health and disease. To further deepen our …
processes with significant relevance in health and disease. To further deepen our …