Recent advances in biocatalysis with chemical modification and expanded amino acid alphabet

AD Pagar, MD Patil, DT Flood, TH Yoo… - Chemical …, 2021 - ACS Publications
The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …

Ultrasound as a pre-treatment for extraction of bioactive compounds and food safety: A review

CO Perera, MAJ Alzahrani - Lwt, 2021 - Elsevier
Application of ultrasound has been considered a “green” technology, because it offers
advantages in terms of selectivity, better quality, use of less chemicals, and energy …

Oxidoreductase-catalyzed synthesis of chiral amines

MD Patil, G Grogan, A Bommarius, H Yun - Acs Catalysis, 2018 - ACS Publications
Chiral amines are valuable constituents of many important pharmaceutical compounds and
their intermediates. It is estimated that∼ 40%–45% of small molecule pharmaceuticals …

Progress in ultrasound-assisted extraction of the value-added products from microorganisms

S Zheng, G Zhang, HJ Wang, Z Long, T Wei… - World Journal of …, 2021 - Springer
Extracting value-added products from microorganisms is an important research focus for the
future. Among the many extraction methods, ultrasound-assisted extraction (UAE) has …

L-arginase from Streptomyces diastaticus MAM5 as a potential therapeutic agent in breast cancer: purification, characterization, G1 phase arrest and autophagy …

AA Hassabo, M Abdelraof, RM Allam - International Journal of Biological …, 2023 - Elsevier
Targeting cancer metabolic processes has increased interest over the last century. Cancer
cells have an enhanced proliferation rate that requires high quantities of amino acids …

Stability of immobilized L-arginine deiminase from Penicillium chrysogenum and evaluation of its anticancer activity

HM El-Shora, GS El-Sayyad, NA El-Zawawy… - Scientific Reports, 2024 - nature.com
The aim of the present work was to immobilize L-arginine deiminase on suitable supports
such as chitosan, alginate, and silica gel to study its stability. Additionally, the study aims to …

Purification and characterization of arginine deiminase from Pseudomonas putida: Structural insights of the differential affinities of l-arginine analogues

MD Patil, VP Rathod, UR Bihade… - Journal of bioscience and …, 2019 - Elsevier
Arginine deiminase (ADI) from Pseudomonas putida was purified using ammonium sulphate
precipitation, anion exchange and hydrophobic interaction chromatography. Influence of …

Kinetic Resolution of Racemic Amines to Enantiopure (S)-amines by a Biocatalytic Cascade Employing Amine Dehydrogenase and Alanine Dehydrogenase

MD Patil, S Yoon, H Jeon, TP Khobragade, S Sarak… - Catalysts, 2019 - mdpi.com
Amine dehydrogenases (AmDHs) efficiently catalyze the NAD (P) H-dependent asymmetric
reductive amination of prochiral carbonyl substrates with high enantioselectivity. AmDH …

Arginine deaminase from Pseudomonas aeruginosa PS2: purification, biochemical characterization and in-vitro evaluation of anticancer activity

K Bala, I Husain, A Sharma - 3 Biotech, 2020 - Springer
In the present study, arginine deaminase (ADI) was purified from Pseudomonas aeruginosa
PS2 which showed relative molecular mass of 70±3 kDa on native-PAGE and 36±0.5 kDa …

破壁方法对红球菌11-3 胞内几丁质脱乙酰酶释放的影响.

肖宇, 石文琪, 于宏伟, 马爱进… - Journal of Chinese …, 2023 - search.ebscohost.com
摘要红球菌11* 3 是< 株高产几丁质脱乙酞酶(CDA) 的菌株, 几丁质在该酶的催化作用下可转化
为壳聚糖, 该酶在壳聚糖的生产中具有重要作用, 红球菌11* 3 菌株所产CDA 为胞内酶 …