Polarization microscopy: from ensemble structural imaging to single-molecule 3D orientation and localization microscopy

S Brasselet, MA Alonso - Optica, 2023 - opg.optica.org
Optical contrasts in microscopy are sensitive to light polarization, whose interaction with
molecular dipoles provides an important lever for probing molecular orientation. Polarization …

Single-Molecule Spectroscopy and Super-Resolution Mapping of Physicochemical Parameters in Living Cells

MA Steves, C He, K Xu - Annual Review of Physical Chemistry, 2024 - annualreviews.org
By superlocalizing the positions of millions of single molecules over many camera frames, a
class of super-resolution fluorescence microscopy methods known as single-molecule …

Biomolecular condensates are characterized by interphase electric potentials

AE Posey, A Bremer, NA Erkamp, A Pant… - Journal of the …, 2024 - ACS Publications
Biomolecular condensates form via processes that combine phase separation and
reversible associations of multivalent macromolecules. Condensates can be two-or …

POLCAM: instant molecular orientation microscopy for the life sciences

E Bruggeman, O Zhang, LM Needham, M Körbel… - Nature …, 2024 - nature.com
Current methods for single-molecule orientation localization microscopy (SMOLM) require
optical setups and algorithms that can be prohibitively slow and complex, limiting …

Three-dimensional dipole orientation mapping with high temporal-spatial resolution using polarization modulation

S Zhong, L Qiao, X Ge, X Xu, Y Fu, S Gao, K Zhanghao… - PhotoniX, 2024 - Springer
Fluorescence polarization microscopy is widely used in biology for molecular orientation
properties. However, due to the limited temporal resolution of single-molecule orientation …

Single-molecule orientation imaging reveals two distinct binding configurations on amyloid fibrils

A Sarkar, V Namboodiri… - The Journal of Physical …, 2023 - ACS Publications
Fluorescence readouts for an amyloid fibril sensor critically depend on its molecular
interaction and local environment offered by the available structural motifs. Here we employ …

Resolving the Nanoscale Structure of β-Sheet Peptide Self-Assemblies Using Single-Molecule Orientation–Localization Microscopy

W Zhou, CL O'Neill, T Ding, O Zhang, JS Rudra… - ACS …, 2024 - ACS Publications
Synthetic peptides that self-assemble into cross-β fibrils are versatile building blocks for
engineered biomaterials due to their modularity and biocompatibility, but their structural and …

Fundamental limits in measuring the anisotropic rotational diffusion of single molecules

W Zhou, T Wu, MD Lew - The Journal of Physical Chemistry A, 2024 - ACS Publications
Many biophysical techniques, such as single-molecule fluorescence correlation
spectroscopy, Förster resonance energy transfer, and fluorescence anisotropy, measure …

Investigating 3D microbial community dynamics of the rhizosphere using quantitative phase and fluorescence microscopy

O Zhang, RE Alcalde, H Zhou, S Yin… - Proceedings of the …, 2024 - pnas.org
Microbial interactions in the rhizosphere contribute to soil health, making understanding
these interactions crucial for sustainable agriculture and ecosystem management. Yet it is …

Fourier imaging for nanophotonics

S Cueff, L Berguiga, HS Nguyen - Nanophotonics, 2024 - degruyter.com
Standard optical characterization and spectroscopy techniques rely on the measurement of
specular reflection, transmission, or emission at normal incidence. Although the usefulness …