Crop improvement by genome editing involves the targeted alteration of genes to improve
plant traits, such as stress tolerance, disease resistance or nutritional content. Techniques …

The development of the clustered regularly interspaced short palindromic repeats/CRISPR-
associated protein (CRISPR/Cas) system has provided precise and efficient strategies to …

CRISPR‐based directed evolution is an effective breeding biotechnology to improve
agronomic traits in plants. However, its gene diversification is still limited using individual …

Background Soybean is a worldwide-cultivated crop due to its applications in the food, feed,
and biodiesel industries. Genome editing in soybean began with ZFN and TALEN …

Summary The Er Cas12a nuclease, also known as MAD7, is part of a CRISPR/Cas system
from Eubacterium rectale and distantly related to Cas12a nucleases. As it shares only 31 …

Background CRISPR/Cas-derived base editor enables precise editing of target sites and
has been widely used for basic research and crop genetic improvement. However, the …

Cytosine base editors (CBEs) and adenine base editors (ABEs) enable precise C-to-T and A-
to-G edits. Recently, ABE8e, derived from TadA-8e, enhances A-to-G edits in mammalian …

CRISPR–Cas technologies contribute to enhancing our understanding of plant gene
functions, and to the precise breeding of crop traits. Here, we review the latest progress in …

A plethora of CRISPR effectors, such as Cas3, Cas9, and Cas12a, are commonly employed
as gene editing tools. Among these, Cas12 effectors developed based on Class II type V …

Mutagenesis driving genetic diversity is vital for understanding and engineering biological
systems. However, the lack of effective methods to generate in-situ mutagenesis in multiple …