The vast majority of genomic sequences are automatically annotated using various software
programs. The accuracy of these annotations depends heavily on the very few manual …

The transcriptional control of sporulation in Bacillus subtilis is reasonably well understood,
but its translational control is underexplored. Here, we use RNA-seq, ribosome profiling and …

Regulation of messenger RNA stability is pivotal for programmed gene expression in
bacteria and is achieved by a myriad of molecular mechanisms. By bulk sequencing of 5 …

The production of the alpha-amylase (AMY) enzyme in Bacillus subtilis at a high rate leads
to the accumulation of unfolded AMY, which causes secretion stress. The over-expression of …

Background Bacillus subtilis is a Gram-positive bacterium used as a cell factory for protein
production. Over the last decades, the continued optimization of production strains has …

Laboratory experimental evolution provides a powerful tool for studying microbial adaptation
to different environments. To understand the differences and similarities of the dynamic …

The bacterial cell wall (CW) is an essential protective barrier and the frontline of cellular
interactions with the environment and also a target for numerous antimicrobial agents …

Yield improvements in cell factories can potentially be obtained by fine-tuning the regulatory
mechanisms for gene candidates. In pursuit of such candidates, we performed RNA …

Accurate annotation to single-nucleotide resolution of the transcribed regions in genomes is
key to optimally analyse RNA-seq data, understand regulatory events and for the design of …

Understanding bacterial gene function remains a major biological challenge. Double-mutant
genetic interaction (GI) analysis addresses this challenge by uncovering the functional …