Recombinant proteins are becoming increasingly important for industrial applications, where
Escherichia coli is the most widely used bacterial host for their production. However, the …

Formation of inclusion bodies in bacterial hosts poses a major challenge for large scale
recovery of bioactive proteins. The process of obtaining bioactive protein from inclusion …

Protein aggregation occurs as a consequence of perturbations in protein homeostasis that
can be triggered by environmental and cellular stresses. The accumulation of protein …

The purpose of this work was to construct a consensus prediction algorithm of 'aggregation-
prone'peptides in globular proteins, combining existing tools. This allows comparison of the …

Bacterial inclusion bodies (IBs) are functional, non-toxic amyloids occurring in recombinant
bacteria showing analogies with secretory granules of the mammalian endocrine system …

Neurodegenerative diseases are featured by progressive dysfunction and death of cells in
selected areas in the nervous system, determining clinical presentation. Neuronal loss is …

Many protein species produced in recombinant bacteria aggregate as insoluble protein
clusters named inclusion bodies (IBs). IBs are discarded from further processing or are …

Understanding the structure, functionalities and biology of functional amyloids is an issue of
emerging interest. Inclusion bodies, namely protein clusters formed in recombinant bacteria …

Understanding the mechanisms underlying protein misfolding and aggregation has become
a central issue in biology and medicine. Compelling evidence show that the formation of …

As unique biopolymers, proteins can be employed for therapeutic delivery. They bear
important features such as bioavailability, biocompatibility, and biodegradability with low …