GTPases of the Ras superfamily regulate many aspects of cell growth, differentiation and
action. Their functions depend on their ability to alternate between inactive and active forms …

Recent advances have uncovered the general protein apparatus used by all eukaryotes for
intracellular transport, including secretion and endocytosis, and for triggered exocytosis of …

A WIDE variety of membrane transformations important in intracellular transport are inhibited
by the fungal metabolite brefeldin A (refs 1–4), implying that the target for this drug is central …

Abstract Synthesis of phosphatidylinositol 4, 5-bisphosphate [PI (4, 5) P 2], a signaling
phospholipid, is primarily carried out by phosphatidylinositol 4-phosphate 5-kinase [PI (4) …

THE fungal metabolite brefeldin A is a powerful tool for investigating membrane traffic in
eukaryotic cells1. The effects of brefeldin A on traffic are partly explained by its ability to …

Eukaryotic life depends on the spatial and temporal organization of cellular membrane
systems. Recent advances in understanding the machinery of vesicle transport have …

Vesicle trafficking is essential for the generation of asymmetries, which are central to
multicellular development. Core components of the vesicle transport machinery, such as …

We have examined the role of ras-related rab proteins in transport from the ER to the Golgi
complex in vivo using a vaccinia recombinant T7 RNA polymerase virus to express site …

The hydrolysis of phosphatidylcholine by phospholipase D (PLD) results in the production of
phosphatidic acid and choline. An assay that uses an exogenous substrate was developed …

Using three different trans dominant mutants of bovine ARF1 affecting GDP exchange or
GTP hydrolysis we demonstrate the central role of ARF1 in controlling vesicular traffic from …