Holographic tomography: techniques and biomedical applications

V Balasubramani, A Kuś, HY Tu, CJ Cheng… - Applied Optics, 2021 - opg.optica.org
Holographic tomography (HT) is an advanced label-free optical microscopic imaging
method used for biological studies. HT uses digital holographic microscopy to record the …

Label-free multiphoton microscopy: much more than fancy images

G Borile, D Sandrin, A Filippi, KI Anderson… - International Journal of …, 2021 - mdpi.com
Multiphoton microscopy has recently passed the milestone of its first 30 years of activity in
biomedical research. The growing interest around this approach has led to a variety of …

Label‐free intracellular multi‐specificity in yeast cells by phase‐contrast tomographic flow cytometry

V Bianco, M D'Agostino, D Pirone, G Giugliano… - Small …, 2023 - Wiley Online Library
In‐flow phase‐contrast tomography provides a 3D refractive index of label‐free cells in
cytometry systems. Its major limitation, as with any quantitative phase imaging approach, is …

Refractive index changes of cells and cellular compartments upon paraformaldehyde fixation acquired by tomographic phase microscopy

M Baczewska, K Eder, S Ketelhut, B Kemper… - Cytometry Part …, 2021 - Wiley Online Library
Three‐dimensional quantitative phase imaging is an emerging method, which provides the
3D distribution of the refractive index (RI) and the dry mass in live and fixed cells as well as …

Influence of Chemical and Genetic Manipulations on Cellular Organelles Quantified by Label-Free Optical Diffraction Tomography

MJ Cho, CE Kim, YH Shin, JK Kim… - Analytical Chemistry, 2023 - ACS Publications
Label-free optical diffraction tomography provides three-dimensional imaging of cells and
organelles, along with their refractive index (RI) and volume. These physical parameters are …

Label-free fiber nanograting sensor for real-time in situ early monitoring of cellular apoptosis

D Li, N Wang, T Zhang, G Wu, Y Xiong… - Advanced …, 2022 - spiedigitallibrary.org
The achievement of functional nanomodules for subcellular label-free measurement has
long been pursued in order to fully understand cellular functions. Here, a compact label-free …

Monitoring Stress Response Difference in Nucleolus Morphology and ATP Content Changes during Hyperthermia Cell Apoptosis with Plasmonic Fluorescent …

X Guan, B Wang, Y Zhang, G Qi, L Chen… - Analytical …, 2022 - ACS Publications
The nucleolus, as a main “cellular stress receptor”, is the hub of the stress response driving
cancer development and has great research value in the field of organelle-targeting …

[HTML][HTML] Characterizing organelles in live stem cells using label-free optical diffraction tomography

Y Kim, TK Kim, Y Shin, E Tak, GW Song, YM Oh… - Molecules and cells, 2021 - Elsevier
Label-free optical diffraction tomography (ODT), an imaging technology that does not require
fluorescent labeling or other pre-processing, can overcome the limitations of conventional …

Mitotic chromosomes in live cells characterized using high-speed and label-free optical diffraction tomography

TK Kim, BW Lee, F Fujii, KH Lee, S Lee, YK Park… - Cells, 2019 - mdpi.com
The cell nucleus is a three-dimensional, dynamic organelle organized into subnuclear
compartments such as chromatin and nucleoli. The structure and function of these …

Nucleolar organization and functions in health and disease

U Stochaj, SC Weber - Cells, 2020 - mdpi.com
The nucleolus is a prominent, membraneless compartment found within the nucleus of
eukaryotic cells. It forms around ribosomal RNA (rRNA) genes, where it coordinates the …