Fluorescence microscopy provides an efficient and unique approach to study fixed and
living cells because of its versatility, specificity, and high sensitivity. Fluorescence …

Synaptic inhibition plays a crucial role in regulating neuronal excitability, which is the
foundation of nervous system function. This inhibition is largely mediated by the …

Chloride and protons perform important closely related roles in many cellular responses.
Here we developed a ratiometric biosensor, ClopHensor, based on a highly chloride …

Fluorescence lifetime imaging microscopy (FLIM) and fluorescence anisotropy imaging
microscopy (FAIM) are versatile tools for the investigation of the molecular environment of …

Fluorescence lifetime imaging (FLIM) and fluorescence polarization imaging are
complementary techniques that can be used to extract information about macromolecules …

Since the physical process of fluorescence resonance energy transfer (FRET) was
elucidated more than six decades ago, this peculiar fluorescence phenomenon has turned …

Time-correlated single photon counting (TCSPC) is a widely used, robust and mature
technique to measure the photon arrival time in applications such as fluorescence …

Chloride homeostasis has a pivotal role in controlling neuronal excitability in the adult brain
and during development. The intracellular concentration of chloride is regulated by the …

The enzymatic activity of the Src family tyrosine kinase p56Lck (Lck) is tightly controlled by
differential phosphorylation of two tyrosine residues, Tyr394 and Tyr505. Phosphorylation of …

The lymphocyte-specific Src family protein tyrosine kinase p56Lck (Lck) is essential for T cell
development and activation and, hence, for adaptive immune responses. The mechanism by …