▪ Abstract The events leading to transcription of eukaryotic protein-coding genes culminate
in the positioning of RNA polymerase II at the correct initiation site. The core promoter, which …

Synthetic control of gene expression is critical for metabolic engineering efforts. Specifically,
precise control of key pathway enzymes (heterologous or native) can help maximize product …

Transcription of the lymphocyte-specific terminal deoxynucleotidyltransferase gene begins
at a single nucleotide, but no TATA box is present. We have identified a 17 bp element that …

We identified a gene of the fungal pathogen Candida albicans, designated EFG1, whose
high‐level expression stimulates pseudohyphal morphogenesis in the yeast …

Transcription initiation by RNA polymerase II (RNA pol II) requires interaction between cis-
acting promoter elements and trans-acting factors. The eukaryotic promoter consists of core …

The nmt1 gene of Schizosaccharomyces pombe is highly expressed and subject to
transcriptional repression by thiamine. The nmt1 promoter, in common with other strong …

The core promoter of eukaryotic genes is the minimal DNA region that recruits the basal
transcription machinery to direct efficient and accurate transcription initiation. The fraction of …

Subunit a of the vacuolar membrane H (+)-translocating adenosine triphosphatase of the
yeast Saccharomyces cerevisiae contains a catalytic site for ATP hydrolysis. N-terminal …

Ubiquitin is a 76‐residue protein highly conserved among eukaryotes. Conjugation of
ubiquitin to intracellular proteins mediates their selective degradation in vivo. We describe a …

The CYC1 gene of Saccharomyces cerevisiae is positively regulated by the HAP2 and
HAP3 proteins, which form a heteromeric complex that binds to a CCAAT box in the …