Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Elemental imaging gives insight into the fundamental chemical makeup of living organisms.
Every cell on Earth is comprised of a complex and dynamic mixture of the chemical elements …

Fluorescence lifetime imaging microscopy (FLIM) is used in diverse disciplines, including
biology, chemistry and biophysics, but its use has been limited by the complexity of the data …

The success of fluorescence experiments requires attention to experimental details and an
understanding of the instrumentation. There are also many potential artifacts that can distort …

Changing the data representation from the classical time delay histogram to the phasor
representation provides a global view of the fluorescence decay at each pixel of an image …

Biomedical photonics is defined as the science of harnessing light and other forms of radiant
energy to address problems in medicine and biology. The field has experienced explosive …

We describe a label-free imaging method to monitor stem-cell metabolism that discriminates
different states of stem cells as they differentiate in living tissues. In this method we use …

Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique offering profound
insights into a broad spectrum of biological processes such as metabolic imaging, protein …

In biological microscopy, the ever expanding range of applications requires quantitative
approaches that analyze several distinct fluorescent molecules at the same time in the same …

Green fluorescence protein (GFP)-based fluorescence resonance energy transfer (FRET) is
increasingly used in investigation of inter-and intramolecular interactions in living cells. In …