Long-term primary culture of mammalian cells has been always difficult due to unavoidable
senescence. Conventional methods for generating immortalized cell lines usually require …

Fluid secretion by exocrine glandular organs is essential to the survival of mammals. Each
glandular unit within the body is uniquely organized to carry out its own specific functions …

Abstract Purpose Meibomian glands (MGs) are crucial for maintaining tear film stability and
ocular surface health. Here, we aim to establish a novel organotypic culture model of MGs …

Age-related meibomian gland dysfunction (MGD) is the main cause of evaporative dry eye
disease in an aging population. Decreased meibocyte cell renewal and lipid synthesis are …

Meibomian glands (MGs) are vital for ocular surface health. However, the roles of
inflammation in the progression of meibomian gland dysfunction (MGD) are largely …

Meibomian gland dysfunction is one of the most common ocular diseases, with therapeutic
treatment being primarily palliative due to our incomplete understanding of meibomian …

In recent decades, the establishment of complex three-dimensional (3D) models of tissues
has allowed researchers to perform high-quality studies and to not only advance knowledge …

Purpose: Meibomian glands play a vital role in maintaining ocular surface stability. This
study aimed to investigate whether Hedgehog signaling is involved in the regulation of …

Meibomian gland dysfunction (MGD) is a chronic abnormality of the Meibomian glands
(MGs) that is recognized as the leading cause of evaporative dry eye worldwide. Despite its …

Purpose: To establish a simplified three-dimensional (3D) meibomian gland culture model
using a meibomian gland epithelial cell (HMGEC) line that might be a useful tool to gain …