Background Gastric cancer is the third leading cause of cancer-related deaths worldwide
and has been associated with infections that may promote tumour progression. Accordingly …

Background Developments in molecular detection and strain differentiation of members of
Mycobacterium tuberculosis complex have proved to be useful. The DNA extraction method …

In this study, we report the molecular diagnosis and retrospective study of porcine circovirus
3 (PCV3) in frozen and formalin-fixed paraffin-embedded swine tissues (FFPE) collected …

Chagas disease in the chronic phase may develop into cardiac and/or digestive forms. The
pathogenesis of the disease is not yet clear and studies have been carried out to elucidate …

Successful DNA extraction is indispensable for molecular methods based on polymerase
chain reaction (PCR); however, goat sperm DNA extraction is limited. Thus, the aim of this …

Standardization of the methods for extraction of DNA from sand flies is essential for
obtaining high efficiency during subsequent molecular analyses, such as the new …

Background Plasmodium vivax is the main species responsible for human malaria in Brazil,
and one of its manifestations is splenic malaria, though there are still challenges in its …

The purpose of the present work was to evaluate the accuracy of quantitative polymerase
chain reaction (qPCR) performed on samples of fresh frozen tissue (FT) and formalin-fixed …

Os avanços tecnológicos e metodológicos da biologia molecular criaram a possibilidade de
obter DNA de amostras teciduais estocadas em blocos de parafina, alternativa esta que …

INTRODUÇÃO: Diversos métodos para extração de DNA a partir de tecidos biológicos
inclusos em parafina encontram-se descritos na literatura, sendo o sucesso desse …