Size exclusion chromatography and asymmetrical flow field-flow fractionation for structural characterization of polysaccharides: A comparative review

Z Chen, D Wang, S Gu, N Wu, K Wang… - International Journal of …, 2024 - Elsevier
Natural polysaccharides exhibit a wide range of biological activities, which are closely
related to their structural characteristics, including their molecular weight distribution, size …

[HTML][HTML] Multi-attribute characterization of pneumococcal conjugate vaccine by Size-exclusion chromatography coupled with UV-MALS-RI detections

JZ Deng, C Lancaster, MA Winters, KM Phillips… - Vaccine, 2022 - Elsevier
Streptococcus pneumoniae bacterial infection can cause serious diseases. Among more
than 90 known streptococcus pneumoniae serotypes, more than 30 can cause invasive …

[HTML][HTML] O-acetylation of typhoid capsular polysaccharide confers polysaccharide rigidity and immunodominance by masking additional epitopes

K Hitri, MM Kuttel, G De Benedetto, K Lockyer, F Gao… - Vaccine, 2019 - Elsevier
In this work, we explore the effects of O-acetylation on the physical and immunological
characteristics of the WHO International Standards of Vi polysaccharide (Vi) from both …

Antibody enhanced HPLC for serotype-specific quantitation of polysaccharides in pneumococcal conjugate vaccine

JZ Deng, N Kuster, A Drumheller, M Lin, F Ansbro… - npj Vaccines, 2023 - nature.com
Bacterial infection remains as one of the major healthcare issues, despite significant
scientific and medical progress in this field. Infection by Streptococcus Pneumoniae (S …

Characterization of high molecular weight pneumococcal conjugate by SEC-MALS and AF4-MALS

JZ Deng, J Lin, M Chen, C Lancaster, P Zhuang - Polymers, 2022 - mdpi.com
Infections by Streptococcus pneumoniae can cause serious pneumococcal diseases and
other medical complications among patients. Polysaccharide-based vaccines have been …

Lessons learned and future challenges in the design and manufacture of glycoconjugate vaccines

JP Hennessey Jr, P Costantino, P Talaga… - … : From Concept to …, 2018 - ACS Publications
Glycoconjugate vaccines have proven to be highly effective in preventing a variety of
bacterial diseases. Publicly available data provides a partial understanding of best practices …

New utilization of Polygonum multiflorum polysaccharide as macromolecular carrier of 5-fluorouracil for controlled release and immunoprotection

Q Zhang, Y Xu, J Lv, M Cheng, Y Wu, K Cao… - International journal of …, 2018 - Elsevier
WPMP-2 is an acid polysaccharide isolated from Polygonum multiflorum, which
demonstrated excellent immunomodulatory activity. In order to reduce immunosuppression …

[HTML][HTML] Multivariable analysis of egg white protein-chitosan interaction: Influence of pH, temperature, biopolymers ratio, and ionic concentration

Y Wang, X Li, N Gao, X Wang, S Sun - Food Chemistry: X, 2023 - Elsevier
The influence of pH, temperature, biopolymer ratio, total concentration, and ionic
concentration on the interaction between egg white protein (EWP) and chitosan (CS) was …

Purification of a conjugated polysaccharide vaccine using tangential flow diafiltration

P Emami, SP Motevalian, E Pepin… - Biotechnology and …, 2019 - Wiley Online Library
Conjugated vaccines prepared from the capsular polysaccharide of Streptococcus
pneumoniae can provide immunization against invasive pneumococcal disease, meningitis …

Identification and quantitation of isomeric pneumococcal polysaccharides by partial chemical degradation followed by mass spectrometry

J Manheim, M Lin, J Kong, M Biba, P Zhuang - Carbohydrate Polymers, 2022 - Elsevier
Quantitation of isomeric pneumococcal polysaccharides in vaccines is a challenging task
due to mixture complexity, their low quantities, and identical monosaccharide compositions …