Since its initial demonstration in 2000, far-field super-resolution light microscopy has
undergone tremendous technological developments. In parallel, these developments have …

Super-resolution fluorescence microscopy allows the investigation of cellular structures at
nanoscale resolution using light. Current developments in super-resolution microscopy have …

High-throughput super-resolution (SR) imaging is attractive for rapid and high-precision
profiling in a wide range of biomedical applications. However, current SR methods require …

Live-cell super-resolution microscopy enables the imaging of biological structure dynamics
below the diffraction limit. Here we present enhanced super-resolution radial fluctuations …

Spectrally separated fluorophores allow the observation of multiple targets simultaneously
inside living cells, leading to a deeper understanding of the molecular interplay that …

Due to the wave nature of light, optical microscopy has a lower‐bound lateral resolution limit
of approximately half of the wavelength of visible light, that is, within the range of 200 to 350 …

Super-resolution microscopy techniques have pushed-down the limits of resolution in optical
imaging by more than an order of magnitude. However, these methods often require long …

3D super-resolution fluorescence microscopy typically requires sophisticated setups,
sample preparation, or long measurements. A notable exception, SOFI, only requires …

The presence of dark states causes fluorescence intermittency of single molecules due to
transitions between “on” and “off” states. Genetically encodable markers such as fluorescent …

摘要超分辨荧光显微成像技术可以突破光学衍射极限, 为研究亚细胞结构乃至蛋白质等生物大
分子的内部动力学过程提供了极其重要的研究工具, 因此自发明以来就被生物及医学领域的科研 …