The function and activity of a protein are often modulated by other proteins with which it
interacts. This review is intended as a practical guide to the analysis of such protein-protein …

Endocytosis, the process whereby the plasma membrane invaginates to form vesicles, is
essential for bringing many substances into the cell and for membrane turnover. The …

We describe a rapid, sensitive process for comprehensively identifying proteins in
macromolecular complexes that uses multidimensional liquid chromatography (LC) and …

In budding yeast, many proteins involved in endocytic internalization, including adaptors
and actin cytoskeletal proteins, are localized to cortical patches of differing protein …

Mutations of the lethal (l) di large-7 (d/g) tumor suppressor gene of Drosophila cause
neoplastic overgrowth of the imaginal discs. Sequencing of a near full-length cDNA predicts …

Recent proteome-wide screening approaches have provided a wealth of information about
interacting proteins in various organisms. To test for a potential association between protein …

We report that the actin assembly inhibitor latrunculin-A (LAT-A) causes complete disruption
of the yeast actin cytoskeleton within 2–5 min, suggesting that although yeast are nonmotile …

Budding in the yeast Saccharomyces cerevisiae involves a polarized deposition of new cell
surface material that is associated with a highly asymmetric disposition of the actin …

All cells undergo rapid remodeling of their actin networks to regulate such critical processes
as endocytosis, cytokinesis, cell polarity, and cell morphogenesis. These events are driven …

The establishment of cell polarity is critical for the development of many organisms and for
the function of many cell types. A large number of studies of diverse organisms from yeast to …