CRISPR–Cas systems provide resistance against foreign mobile genetic elements and have
a wide range of genome editing and biotechnological applications. In this Review, we …

Clustered regulatory interspaced short palindromic repeats (CRISPR) has changed
biomedical research and provided entirely new models to analyze every aspect of …

CRISPR/Cas system is now playing an important role in biosensing applications, especially
when the trans cleavage activity of several Cas effectors is discovered. Recently …

Existing CRISPR/cas-based biosensors usually improve sensitivity by target amplification,
which is time-consuming and susceptible to impurities in complex biofluid. Herein, this is the …

CRISPR/Cas machinery has revolutionized the analytical platform for the precise detection
of diverse analytes. Specific target recognition and robust signal transduction are crucial …

CRISPR-Cas12a is a powerful platform for DNA-based diagnostics. The detection scheme
relies on unselective shredding of a fluorescent ssDNA reporter upon target DNA …

The first genetically modified large animals were developed in 1985 by microinjection to
increase the growth of agricultural livestock such as pigs. Since then, it has been a difficult …

In the ten years since the discovery of the first anti-CRISPR (Acr) proteins, the number of
validated Acrs has expanded rapidly, as has our understanding of the diverse mechanisms …

The sensitive and accurate detection of rare mutations has profound clinical implications;
however, current methods require expensive instrumentation and are laborious and time …

The CRISPR-Cas12a nuclease shreds short single-stranded DNA (ssDNA) substrates
indiscriminately through trans-cleavage upon activation with a specific target DNA. This …