In the quest for better medicines, attention is increasingly turning to cell-based therapies.
The rationale is that infused cells can provide a targeted therapy to precisely correct a …

Intracellular ice formation (IIF) has been linked to death of cells cryopreserved in
suspension. It has been assumed that cells can be supercooled by 2 to 10° C before IIF …

Cryopreservation is the use of very low subzero temperatures to preserve cells and tissues
for later use. This is achieved by controlled cooling in the presence of cryoprotectants that …

Cellular response during the freeze–thaw process strongly affects the cryopreservation
outcome including cell morphology and cell viability. Cryomicroscopy was used to …

Cryopreservation currently is the only method for long-term preservation of cellular viability
and function for uses in cellular therapies. Characterizing the cryobiological response of a …

The isolation of undifferentiated adult stem/progenitor cells remains a challenging task
primarily due to the rare quantity of these cells in biological samples and the lack of unique …

Cryopreservation is the storage of biological matter at subzero temperatures to preserve it.
As one can imagine, this comes with many challenges, including exposure to high solute …

A novel model capable of quantitatively describing and predicting intracellular ice formation
(IIF) as a function of temperature in a cell population characterized by a size distribution is …

It has been hypothesized that pores in the plasma membrane form under conditions of rapid
water efflux, allowing extracellular ice to grow into the cytoplasm under conditions of rapid …

We have investigated the confounding effects of dynamic range limitations on measurement
of the osmotically inactive volume using electrical sensing zone instruments (eg, Coulter …