Abstract Here we present Primer3Plus, a new web interface to the popular Primer3 primer design program as an enhanced alternative for the CGI-scripts that come with Primer3 …
A Spandidos, X Wang, H Wang… - Nucleic acids research, 2010 - academic.oup.com
ABSTRACT PrimerBank (http://pga. mgh. harvard. edu/primerbank/) is a public resource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and …
Background Medium-to large-scale expression profiling using quantitative polymerase chain reaction (qPCR) assays are becoming increasingly important in genomics research. A major …
A Spandidos, X Wang, H Wang, S Dragnev, T Thurber… - BMC genomics, 2008 - Springer
Background Quantitative polymerase chain reaction (QPCR) is a widely applied analytical method for the accurate determination of transcript abundance. Primers for QPCR have …
PK Wolber, PJ Collins, AB Lucas, A De Witte… - Methods in …, 2006 - Elsevier
Microarray technology has become a standard tool in many laboratories. Agilent Technologies manufactures a variety of catalog and custom long‐oligonucleotide (60‐mer) …
High throughput genotyping is essential for studying the spread of multiple antimicrobial resistance. A test oligonucleotide microarray designed to detect 94 antimicrobial resistance …
DP Kreil, RR Russell, S Russell - Methods in enzymology, 2006 - Elsevier
Oligonucleotide probes are increasingly the method of choice for many modern DNA microarray applications. They provide higher target specificity, probe selection gives …
AM Phillippy, JA Mason, K Ayanbule… - PLoS computational …, 2007 - journals.plos.org
DNA signatures are nucleotide sequences that can be used to detect the presence of an organism and to distinguish that organism from all other species. Here we describe Insignia …
KL Brigand, R Russell, C Moreilhon… - Nucleic Acids …, 2006 - academic.oup.com
Two collections of oligonucleotides have been designed for preparing pangenomic human and mouse microarrays. A total of 148 993 and 121 703 oligonucleotides were designed …