Nucleic acids have been among the first targets for antitumor drugs and antibiotics. With the
unveiling of new biological roles in regulation of gene expression, specific DNA and RNA …

As interests increase in oligonucleotide therapeutics, there has been a greater need for
analytical techniques to properly analyze and quantitate these biomolecules. This article …

Organisms from all domains of life invest a substantial amount of energy for the introduction
of RNA modifications into nearly all transcripts studied to date. Instrumental analysis of RNA …

Since 2016, eight new oligonucleotide therapies have been approved which has led to
increased interest in oligonucleotide analysis. There is a particular need for powerful …

Abstract NanoCluster Beacons (NCBs) are multicolor silver nanocluster probes whose
fluorescence can be activated or tuned by a proximal DNA strand called the activator. While …

Oligonucleotide characterization is a rapidly advancing field in the biopharmaceutical
industry. Understanding critical quality attributes, such as intact mass and impurities …

There has been a significant increase in the use of hydrophilic interaction liquid
chromatography (HILIC) to separate oligonucleotides. This rise in the use of HILIC has …

Improving the mobile phase of electrospray oligonucleotides has been a major focus in the
field of oligonucleotides. These improved mobile phases should reduce the charge state …

Argonaute (Ago) proteins mediate target recognition guiding miRNA to bind complementary
mRNA primarily in the seed region. However, additional pairing can occur beyond the seed …

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is the gold-standard
technique for the discovery, detection and structural characterization of post-transcriptional …