Visualizing and discovering cellular structures with super-resolution microscopy
Super-resolution microscopy has overcome a long-held resolution barrier—the diffraction
limit—in light microscopy and enabled visualization of previously invisible molecular details …
limit—in light microscopy and enabled visualization of previously invisible molecular details …
Three-dimensional localization of single molecules for super-resolution imaging and single-particle tracking
Single-molecule super-resolution fluorescence microscopy and single-particle tracking are
two imaging modalities that illuminate the properties of cells and materials on spatial scales …
two imaging modalities that illuminate the properties of cells and materials on spatial scales …
Small RNAs in bacteria and archaea: who they are, what they do, and how they do it
EGH Wagner, P Romby - Advances in genetics, 2015 - Elsevier
Small RNAs are ubiquitously present regulators in all kingdoms of life. Most bacterial and
archaeal small RNAs (sRNAs) act by antisense mechanisms on multiple target mRNAs …
archaeal small RNAs (sRNAs) act by antisense mechanisms on multiple target mRNAs …
Global mapping of small RNA-target interactions in bacteria
Small RNAs (sRNAs) associated with the RNA chaperon protein Hfq are key
posttranscriptional regulators of gene expression in bacteria. Deciphering the sRNA-target …
posttranscriptional regulators of gene expression in bacteria. Deciphering the sRNA-target …
Hfq: the flexible RNA matchmaker
TB Updegrove, A Zhang, G Storz - Current opinion in microbiology, 2016 - Elsevier
Highlights•Hfq contacts RNA via proximal, distal, rim and C-terminal surfaces.•RNAs interact
with Hfq in multiple ways.•Mechanisms of Hfq-facilitated RNA pairing are being elucidated …
with Hfq in multiple ways.•Mechanisms of Hfq-facilitated RNA pairing are being elucidated …
Optimal stochastic restart renders fluctuations in first passage times universal
S Reuveni - Physical review letters, 2016 - APS
Stochastic restart may drastically reduce the expected run time of a computer algorithm,
expedite the completion of a complex search process, or increase the turnover rate of an …
expedite the completion of a complex search process, or increase the turnover rate of an …
New aspects of RNA-based regulation by Hfq and its partner sRNAs
K Kavita, F de Mets, S Gottesman - Current opinion in microbiology, 2018 - Elsevier
Highlights•Novel cross-linking and sequencing methods uncover new sRNAs and sRNA
networks.•Many sRNAs are encoded within the 3′ and 5′ UTRs of mRNA as well as …
networks.•Many sRNAs are encoded within the 3′ and 5′ UTRs of mRNA as well as …
RNA-based recognition and targeting: sowing the seeds of specificity
RNA is involved in the regulation of multiple cellular processes, often by forming sequence-
specific base pairs with cellular RNA or DNA targets that must be identified among the large …
specific base pairs with cellular RNA or DNA targets that must be identified among the large …
Bioorthogonal masked acylating agents for proximity-dependent RNA labelling
RNA localization is highly regulated, with subcellular organization driving context-
dependent cell physiology. Although proximity-based labelling technologies that use highly …
dependent cell physiology. Although proximity-based labelling technologies that use highly …
Kinetics of dCas9 target search in Escherichia coli
How fast can a cell locate a specific chromosomal DNA sequence specified by a single
stranded oligonucleotide? To address this question we study the CRISPR-associated …
stranded oligonucleotide? To address this question we study the CRISPR-associated …