Optical microscopy has for centuries been a key tool to study living cells with minimum
invasiveness. The advent of single molecule techniques over the past two decades has …

Anisotropic gold nanoparticles (AuNPs) have attracted the interest of scientists for over a
century, but research in this field has considerably accelerated since 2000 with the synthesis …

We review the use of luminescent nanoparticles in super-resolution imaging and single-
molecule tracking, and showcase novel approaches to super-resolution imaging that …

Nanobodies are the recombinant variable domains of heavy-chain-only antibodies, with
many unique properties such as small size, excellent solubility, superior stability, quick …

Since the serendipitous discovery 20 years ago of bona fide camelid heavy-chain
antibodies, their single-domain antigen-binding fragments, known as VHHs or nanobodies …

Nanobodies can be seen as next‐generation tools for the recognition and modulation of
antigens that are inaccessible to conventional antibodies. Due to their compact structure and …

Antibodies are key reagents to investigate cellular processes. The development of
recombinant antibodies and binders derived from natural protein scaffolds has expanded …

Owing to peculiar properties of nanobody, including nanoscale size, robust structure, stable
and soluble behaviors in aqueous solution, reversible refolding, high affinity and specificity …

Actin filaments generate mechanical forces that drive membrane movements during
trafficking, endocytosis and cell migration. Reciprocally, adaptations of actin networks to …

The photothermal (PT) signal arises from slight changes of the index of refraction in a
sample due to absorption of a heating light beam. Refractive index changes are measured …