Over 500 natural and synthetic amino acids have been genetically encoded in the last two
decades. Incorporating these noncanonical amino acids into proteins enables many …

Since the establishment of site-specific mutagenesis of single amino acids to interrogate
protein function in the 1970s, biochemists have sought to tailor protein structure in the native …

Monosubstituted tetrazines are important bioorthogonal reactive tools due to their rapid
ligation with trans-cyclooctene. However, their application is limited by the reactivity–stability …

Genetic code expansion allows unnatural (non-canonical) amino acid incorporation into
proteins of interest by repurposing the cellular translation machinery. The development of …

Conspectus Expansion of the genetic code allows unnatural amino acids (Uaas) to be site-
specifically incorporated into proteins in live biological systems, thus enabling novel …

The genetic code of mammalian cells can be expanded to allow the incorporation of non-
canonical amino acids (ncAAs) by suppressing in-frame amber stop codons (UAG) with an …

The use of light to control protein function is a critical tool in chemical biology. Here we
describe the addition of a photocaged histidine to the genetic code. This unnatural amino …

Unnatural amino acids (UAAs) have gained significant attention in protein engineering and
drug development owing to their ability to introduce new chemical functionalities to proteins …

The genetic code specifies 20 common amino acids and is largely preserved in both single
and multicellular organisms. Unnatural amino acids (Uaas) have been genetically …

The ability to quantify the local electrostatic environment of proteins and protein/peptide
assemblies is key to gaining a microscopic understanding of many biological interactions …