Advances in ultrahigh-throughput screening for directed enzyme evolution

U Markel, KD Essani, V Besirlioglu, J Schiffels… - Chemical Society …, 2020 - pubs.rsc.org
Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …

New Insights into the Cooperativity and Dynamics of Dimeric Enzymes

KW Chen, TY Sun, YD Wu - Chemical Reviews, 2023 - ACS Publications
A survey of protein databases indicates that the majority of enzymes exist in oligomeric
forms, with about half of those found in the UniProt database being homodimeric …

High-throughput screening, next generation sequencing and machine learning: advanced methods in enzyme engineering

R Vanella, G Kovacevic, V Doffini… - Chemical …, 2022 - pubs.rsc.org
Enzyme engineering is an important biotechnological process capable of generating
tailored biocatalysts for applications in industrial chemical conversion and biopharma …

Antioxidant activity of synthetic polymers of phenolic compounds

S Nagarajan, R Nagarajan, J Kumar, A Salemme… - Polymers, 2020 - mdpi.com
In recent years, developing potent antioxidants has been a very active area of research. In
this context, phenolic compounds have been evaluated for their antioxidant activity …

Droplet flow cytometry for single-cell analysis

M Li, H Liu, S Zhuang, K Goda - RSC advances, 2021 - pubs.rsc.org
The interrogation of single cells has revolutionised biology and medicine by providing
crucial unparalleled insights into cell-to-cell heterogeneity. Flow cytometry (including …

Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies

J Jiang, G Yang, F Ma - Biotechnology advances, 2023 - Elsevier
Fluorescence-activated droplet sorting (FADS) has emerged as a powerful tool for ultrahigh-
throughput screening of enzymes, metabolites, and antibodies. Fluorescence coupling …

Directed evolution for enzyme development in biocatalysis

S Gargiulo, P Soumillion - Current opinion in chemical biology, 2021 - Elsevier
As an important sector of the chemical industry, biocatalysis requires the continuous
development of enzymes with tailor-made activity, selectivity, stability, or tolerance to …

[HTML][HTML] Recent advancements in enzyme-mediated crosslinkable hydrogels: In vivo-mimicking strategies

W Song, J Ko, YH Choi, NS Hwang - APL bioengineering, 2021 - pubs.aip.org
Enzymes play a central role in fundamental biological processes and have been traditionally
used to trigger various processes. In recent years, enzymes have been used to tune …

Tissue engineering modalities in skeletal muscles: focus on angiogenesis and immunomodulation properties

AR Namjoo, FN Abrbekoh, S Saghati, H Amini… - Stem Cell Research & …, 2023 - Springer
Muscular diseases and injuries are challenging issues in human medicine, resulting in
physical disability. The advent of tissue engineering approaches has paved the way for the …

Engineering of therapeutic and detoxifying enzymes

F Michailidou - Angewandte Chemie International Edition, 2023 - Wiley Online Library
Therapeutic enzymes present excellent opportunities for the treatment of human disease,
modulation of metabolic pathways and system detoxification. However, current use of …