Light-sheet fluorescent microscopy: fundamentals, developments and applications

H Kafian, S Mozaffari-Jovin, M Bagheri… - Physica …, 2023 - iopscience.iop.org
Deep volumetric microscopy of live objects plays a critical role in biology and medicine. To
this end, development of rapid and non-invasive optical methods for 3-dimensional (3D) …

Frontiers in artificial intelligence‐directed light‐sheet microscopy for uncovering biological phenomena and multiorgan imaging

E Zhu, YR Li, S Margolis, J Wang, K Wang, Y Zhang… - View, 2024 - Wiley Online Library
Light‐sheet fluorescence microscopy (LSFM) introduces fast scanning of biological
phenomena with deep photon penetration and minimal phototoxicity. This advancement …

Airy beam light sheet microscopy boosted by deep learning deconvolution

A Stockhausen, JE Rodriguez-Gatica, J Schweihoff… - Optics …, 2023 - opg.optica.org
Common light sheet microscopy comes with a trade-off between light sheet width defining
the optical sectioning and the usable field of view arising from the divergence of the …

Evaluating the resolution of conventional optical microscopes through point spread function measurement

W Hou, Y Wei - Iscience, 2023 - cell.com
In the imaging process of conventional optical microscopy, the primary factor hindering
microscope resolution is the energy diffusion of incident light, most directly described by the …

3D exploration of gene expression in chicken embryos through combined RNA fluorescence in situ hybridization, immunofluorescence, and clearing

M André, S Dinvaut, V Castellani, J Falk - BMC biology, 2024 - Springer
Background Fine characterization of gene expression patterns is crucial to understand many
aspects of embryonic development. The chicken embryo is a well-established and valuable …

Fluorescent reporters, imaging, and artificial intelligence toolkits to monitor and quantify autophagy, heterophagy, and lysosomal trafficking fluxes

M Rudinskiy, D Morone, M Molinari - Traffic, 2024 - Wiley Online Library
Lysosomal compartments control the clearance of cell‐own material (autophagy) or of
material that cells endocytose from the external environment (heterophagy) to warrant …

Impact of axial chromatic aberration on color-multiplexed differential phase contrast microscopy: A quantitative study

H Liu, J Liu, W Zhou, B Xu, D Xiong, X Yang - Optics and Lasers in …, 2025 - Elsevier
Color-multiplexed differential phase contrast (cDPC) imaging relies on deconvolving phase
gradient images with phase transfer function (PTF) to extract quantitative phase information …

Super resolution reconstruction of fluorescence microscopy images by a convolutional network with physical priors

Q Cai, J Lu, W Gu, D Xiao, B Li, L Xu, Y Gu… - Biomedical Optics …, 2024 - opg.optica.org
Super-solution fluorescence microscopy, such as single-molecule localization microscopy
(SMLM), is effective in observing subcellular structures and achieving excellent …

SeidelNet: an aberration-informed deep learning model for spatially varying deblurring

E Whang, D McAllister, A Reddy… - AI and Optical Data …, 2023 - spiedigitallibrary.org
Practical imaging systems form images with spatially-varying blur, making it challenging to
deblur them and recover critical scene features. To address such systems, we introduce …

Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy

C Taneja, JG George, S Corsetti… - Biomedical Optics …, 2024 - opg.optica.org
The Bessel beam (BB) has found widespread adoption in various forms of light-sheet
microscopy. However, for one-photon fluorescence, the transverse profile of the beam poses …