Recent developments in bioprocessing of recombinant proteins: expression hosts and process development

NK Tripathi, A Shrivastava - Frontiers in Bioengineering and …, 2019 - frontiersin.org
Infectious diseases, along with cancers, are among the main causes of death among
humans worldwide. The production of therapeutic proteins for treating diseases at large …

Exploring the costs of horizontal gene transfer

DA Baltrus - Trends in ecology & evolution, 2013 - cell.com
Horizontal gene transfer (HGT) is one of the most important evolutionary forces within
microbial populations. Although evidence for beneficial fitness effects of HGT is …

Metabolic engineering of yeast for production of fuels and chemicals

J Nielsen, C Larsson, A van Maris, J Pronk - Current opinion in …, 2013 - Elsevier
Microbial production of fuels and chemicals from renewable carbohydrate feedstocks offers
sustainable and economically attractive alternatives to their petroleum-based production …

[PDF][PDF] Engineering tolerance to industrially relevant stress factors in yeast cell factories

Q Deparis, A Claes, MR Foulquié-Moreno… - FEMS yeast …, 2017 - academic.oup.com
The main focus in development of yeast cell factories has generally been on establishing
optimal activity of heterologous pathways and further metabolic engineering of the host …

Plasmid-mediated bioaugmentation for the bioremediation of contaminated soils

C Garbisu, O Garaiyurrebaso, L Epelde… - Frontiers in …, 2017 - frontiersin.org
Bioaugmentation, or the inoculation of microorganisms (eg, bacteria harboring the required
catabolic genes) into soil to enhance the rate of contaminant degradation, has great …

How to achieve high-level expression of microbial enzymes: strategies and perspectives

L Liu, H Yang, H Shin, RR Chen, J Li, G Du… - …, 2013 - Taylor & Francis
Microbial enzymes have been used in a large number of fields, such as chemical,
agricultural and biopharmaceutical industries. The enzyme production rate and yield are the …

Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica

M Vandermies, P Fickers - Microorganisms, 2019 - mdpi.com
Recombinant protein production represents a multibillion-dollar market. Therefore, it
constitutes an important research field both in academia and industry. The use of yeast as a …

Burden imposed by heterologous protein production in two major industrial yeast cell factories: Identifying sources and mitigation strategies

LLB Kastberg, R Ard, MK Jensen… - Frontiers in Fungal …, 2022 - frontiersin.org
Production of heterologous proteins, especially biopharmaceuticals and industrial enzymes,
in living cell factories consumes cellular resources. Such resources are reallocated from …

[HTML][HTML] Simultaneous secretion of seven lignocellulolytic enzymes by an industrial second-generation yeast strain enables efficient ethanol production from multiple …

A Claes, Q Deparis, MR Foulquié-Moreno… - Metabolic …, 2020 - Elsevier
A major hurdle in the production of bioethanol with second-generation feedstocks is the high
cost of the enzymes for saccharification of the lignocellulosic biomass into fermentable …

Non-mammalian eukaryotic expression systems yeast and fungi in the production of biologics

M Garvey - Journal of Fungi, 2022 - mdpi.com
Biologics have become an important area of medical research generating therapeutics
essential for the treatment of many disease states. Biologics are defined as biologically …