Best practices in metabarcoding of fungi: from experimental design to results

L Tedersoo, M Bahram, L Zinger, RH Nilsson… - Molecular …, 2022 - Wiley Online Library
The development of high‐throughput sequencing (HTS) technologies has greatly improved
our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems …

Mycobiome diversity: high-throughput sequencing and identification of fungi

RH Nilsson, S Anslan, M Bahram… - Nature Reviews …, 2019 - nature.com
Fungi are major ecological players in both terrestrial and aquatic environments by cycling
organic matter and channelling nutrients across trophic levels. High-throughput sequencing …

High-level classification of the Fungi and a tool for evolutionary ecological analyses

L Tedersoo, S Sánchez-Ramírez, U Koljalg, M Bahram… - Fungal diversity, 2018 - Springer
High-throughput sequencing studies generate vast amounts of taxonomic data. Evolutionary
ecological hypotheses of the recovered taxa and Species Hypotheses are difficult to test due …

DNA metabarcoding—Need for robust experimental designs to draw sound ecological conclusions

L Zinger, A Bonin, IG Alsos, M Bálint, H Bik… - Molecular …, 2019 - Wiley Online Library
DNA metabarcoding, especially when coupled with high‐through‐put DNA sequencing, is
currently revolutionizing our capacity to assess biodiversity across a full range of taxa and …

Algorithm for post-clustering curation of DNA amplicon data yields reliable biodiversity estimates

TG Frøslev, R Kjøller, HH Bruun, R Ejrnæs… - Nature …, 2017 - nature.com
DNA metabarcoding is promising for cost-effective biodiversity monitoring, but reliable
diversity estimates are difficult to achieve and validate. Here we present and validate a …

Correlation and association analyses in microbiome study integrating multiomics in health and disease

Y Xia - Progress in molecular biology and translational …, 2020 - Elsevier
Correlation and association analyses are one of the most widely used statistical methods in
research fields, including microbiome and integrative multiomics studies. Correlation and …

Rhea: a transparent and modular R pipeline for microbial profiling based on 16S rRNA gene amplicons

I Lagkouvardos, S Fischer, N Kumar, T Clavel - PeerJ, 2017 - peerj.com
The importance of 16S rRNA gene amplicon profiles for understanding the influence of
microbes in a variety of environments coupled with the steep reduction in sequencing costs …

A critical review on exploiting the pharmaceutical potential of plant endophytic fungi

S Gupta, P Chaturvedi, MG Kulkarni… - Biotechnology advances, 2020 - Elsevier
The escalating demand for secondary metabolites in international markets poses a severe
threat to many plant species. An unscrupulous collection is also the immediate challenge to …

The Global Soil Mycobiome consortium dataset for boosting fungal diversity research

L Tedersoo, V Mikryukov, S Anslan, M Bahram… - Fungal Diversity, 2021 - Springer
Fungi are highly important biotic components of terrestrial ecosystems, but we still have a
very limited understanding about their diversity and distribution. This data article releases a …

PacBio metabarcoding of Fungi and other eukaryotes: errors, biases and perspectives

L Tedersoo, A Tooming‐Klunderud… - New Phytologist, 2018 - Wiley Online Library
Second‐generation, high‐throughput sequencing methods have greatly improved our
understanding of the ecology of soil microorganisms, yet the short barcodes (< 500 bp) …