RNA-guided programmable nucleases from CRISPR systems generate precise breaks in
DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA …

CRISPR-based genome editing has been widely implemented in various cell types. In silico
single guide RNA (sgRNA) design is a key step for successful gene editing using the …

Induced pluripotent stem cell (iPSC)-derived organoids provide models to study human
organ development. Single-cell transcriptomics enable highly resolved descriptions of cell …

Self-organizing neural organoids grown from pluripotent stem cells,–combined with single-
cell genomic technologies provide opportunities to examine gene regulatory networks …

To the Editor—The field of genome editing is advancing rapidly1, most recently exemplified
by the advent of base editors that enable changing single nucleotides in a predictable …

CRISPOR. org is a web tool for genome editing experiments with the CRISPR–Cas9 system.
It finds guide RNAs in an input sequence and ranks them according to different scores that …

In the human malaria vector Anopheles gambiae, the gene doublesex (Agdsx) encodes two
alternatively spliced transcripts, dsx-female (AgdsxF) and dsx-male (AgdsxM), that control …

Abstract Homology-directed repair (HDR), a method for repair of DNA double-stranded
breaks can be leveraged for the precise introduction of mutations supplied by synthetic DNA …

The diverse malignant, stromal, and immune cells in tumors affect growth, metastasis, and
response to therapy. We profiled transcriptomes of∼ 6,000 single cells from 18 head and …

Re-expression of the paralogous γ-globin genes (HBG1/2) could be a universal strategy to
ameliorate the severe β-globin disorders sickle cell disease (SCD) and β-thalassemia by …