Base editing: precision chemistry on the genome and transcriptome of living cells
RNA-guided programmable nucleases from CRISPR systems generate precise breaks in
DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA …
DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA …
Lineage recording in human cerebral organoids
Induced pluripotent stem cell (iPSC)-derived organoids provide models to study human
organ development. Single-cell transcriptomics enable highly resolved descriptions of cell …
organ development. Single-cell transcriptomics enable highly resolved descriptions of cell …
Analysis of cardiac myocyte maturation using CASAAV, a platform for rapid dissection of cardiac myocyte gene function in vivo
Rationale: Loss-of-function studies in cardiac myocytes (CMs) are currently limited by the
need for appropriate conditional knockout alleles. The factors that regulate CM maturation …
need for appropriate conditional knockout alleles. The factors that regulate CM maturation …
Inferring and perturbing cell fate regulomes in human brain organoids
Self-organizing neural organoids grown from pluripotent stem cells,–combined with single-
cell genomic technologies provide opportunities to examine gene regulatory networks …
cell genomic technologies provide opportunities to examine gene regulatory networks …
CRISPResso2 provides accurate and rapid genome editing sequence analysis
To the Editor—The field of genome editing is advancing rapidly1, most recently exemplified
by the advent of base editors that enable changing single nucleotides in a predictable …
by the advent of base editors that enable changing single nucleotides in a predictable …
Efficient high-precision homology-directed repair-dependent genome editing by HDRobust
S Riesenberg, P Kanis, D Macak, D Wollny… - Nature …, 2023 - nature.com
Abstract Homology-directed repair (HDR), a method for repair of DNA double-stranded
breaks can be leveraged for the precise introduction of mutations supplied by synthetic DNA …
breaks can be leveraged for the precise introduction of mutations supplied by synthetic DNA …
CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens
JP Concordet, M Haeussler - Nucleic acids research, 2018 - academic.oup.com
CRISPOR. org is a web tool for genome editing experiments with the CRISPR–Cas9 system.
It finds guide RNAs in an input sequence and ranks them according to different scores that …
It finds guide RNAs in an input sequence and ranks them according to different scores that …
[HTML][HTML] A CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes
In the human malaria vector Anopheles gambiae, the gene doublesex (Agdsx) encodes two
alternatively spliced transcripts, dsx-female (AgdsxF) and dsx-male (AgdsxM), that control …
alternatively spliced transcripts, dsx-female (AgdsxF) and dsx-male (AgdsxM), that control …
[HTML][HTML] Single-cell transcriptomic analysis of primary and metastatic tumor ecosystems in head and neck cancer
The diverse malignant, stromal, and immune cells in tumors affect growth, metastasis, and
response to therapy. We profiled transcriptomes of∼ 6,000 single cells from 18 head and …
response to therapy. We profiled transcriptomes of∼ 6,000 single cells from 18 head and …
Long-term evaluation of AAV-CRISPR genome editing for Duchenne muscular dystrophy
Duchenne muscular dystrophy (DMD) is a monogenic disorder and a candidate for
therapeutic genome editing. There have been several recent reports of genome editing in …
therapeutic genome editing. There have been several recent reports of genome editing in …