The traditional two-dimensional (2D) in vitro cell culture system (on a flat support) has long
been used in cancer research. However, this system cannot be fully translated into clinical …

Cell culture is an important and necessary process in drug discovery, cancer research, as
well as stem cell study. Most cells are currently cultured using two-dimensional (2D) …

Abstract The three-dimensional (3D) multicellular tumor spheroids (MCTs) model is
becoming an essential tool in cancer research as it expresses an intermediate complexity …

Three‐dimensional cell culture models, such as spheroids, can be used in the process of the
development of new anticancer agents because they are able to closely mimic the main …

Over the last decades, alginates, natural multifunctional polymers, have increasingly drawn
attention as attractive compounds in the biomedical and pharmaceutical fields due to their …

Cell cultures are important material of study for the variety of advantages that they offer. Both
established continuous cell lines and primary cell cultures continue to be invaluable for …

Of all biologic matrices, decellularized extracellular matrix (dECM) has emerged as a
promising tool used either alone or when combined with other biologics in the fields of tissue …

Cancer is a multifaceted pathology, where cellular and acellular players interact to drive
cancer progression and, in the worst-case, metastasis. The current methods to investigate …

Novel effective treatment is direly needed for patients with pancreatic ductal
adenocarcinoma (PDAC). Therapeutics that target the driver mutations, especially the KRAS …

This review compiles information regarding the use of alginate, and in particular alginate
hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and …