Directed evolution has proved to be an effective strategy for improving or altering the activity
of biomolecules for industrial, research and therapeutic applications. The evolution of …

Over the past ten years, scientific and technological advances have established biocatalysis
as a practical and environmentally friendly alternative to traditional metallo-and …

The amino acid sequence of a protein affects both its structure and its function. Thus, the
ability to modify the sequence, and hence the structure and activity, of individual proteins in …

Microfluidics and novel lab-on-a-chip applications have the potential to boost
biotechnological research in ways that are not possible using traditional methods. Although …

Enzyme engineering is an important biotechnological process capable of generating
tailored biocatalysts for applications in industrial chemical conversion and biopharma …

Bacterial surface display entails the presentation of recombinant proteins or peptides on the
surface of bacterial cells. Escherichia coli is the most frequently used bacterial host for …

Over the past years, technological and scientific advances have proven biocatalysis as a
sustainable alternative than traditional chemical catalysis including organo-or …

Natural evolution relies on the improvement of biological entities by rounds of diversification
and selection. In the laboratory, directed evolution has emerged as a powerful tool for the …

Exploring the sequence space of enzyme catalysts is ultimately a numbers game. Ultrahigh-
throughput screening methods for rapid analysis of millions of variants are therefore …

Directed enzyme evolution has proven to be a powerful tool for improving a range of
properties of enzymes through consecutive rounds of diversification and selection. However …