The survival of vertebrate organisms depends on highly regulated delivery of oxygen and
nutrients through vascular networks that pervade nearly all tissues in the body …

Visualization of the cellular heterogeneity and spatial architecture of the tumor
microenvironment (TME) is becoming increasingly important to understand mechanisms of …

A central challenge in biology is obtaining high-content, high-resolution information while
analyzing tissue samples at volumes relevant to disease progression. We address this here …

Imaging the transcriptome in situ with high accuracy has been a major challenge in single-
cell biology, which is particularly hindered by the limits of optical resolution and the density …

State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact
tissues from individual organs and even to some entire mammals. When combined with light …

Tissue clearing of gross anatomical samples was first described more than a century ago
and has only recently found widespread use in the field of microscopy. This renaissance has …

Abstract The Human Developmental Cell Atlas (HDCA) initiative, which is part of the Human
Cell Atlas, aims to create a comprehensive reference map of cells during development. This …

In oncology, technologies for clinical molecular imaging are used to diagnose patients,
establish the efficacy of treatments and monitor the recurrence of disease. Multiplexed …

Whole-body imaging techniques play a vital role in exploring the interplay of physiological
systems in maintaining health and driving disease. We introduce wildDISCO, a new …

Adeno-associated viruses (AAVs) are commonly used for in vivo gene transfer.
Nevertheless, AAVs that provide efficient transduction across specific organs or cell …