RNA polymerase (RNAP) is a complex molecular machine that governs gene expression
and its regulation in all cellular organisms. To accomplish its function of accurately …

Multi-subunit DNA-dependent RNA polymerases synthesize all classes of cellular RNAs,
ranging from short regulatory transcripts to gigantic messenger RNAs. RNA polymerase has …

The transcription elongation factor TFIIS induces mRNA cleavage by enhancing the intrinsic
nuclease activity of RNA polymerase (Pol) II. We have diffused TFIIS into Pol II crystals and …

RNA chain elongation is a highly processive and accurate process that is finely regulated by
numerous intrinsic and extrinsic signals. Here we describe a general mechanism that …

Structural, biochemical, and genetic studies have led to proposals that a mobile element of
multisubunit RNA polymerases, the Trigger Loop (TL), plays a critical role in catalysis and …

In the cell, RNA polymerase II (pol II) efficiently transcribes DNA packaged into
nucleosomes, but in vitro encounters with the nucleosomes induce catalytic inactivation …

The antibacterial peptide microcin J25 (MccJ25) inhibits transcription by bacterial RNA
polymerase (RNAP). Biochemical results indicate that inhibition of transcription occurs at the …

Transcriptional pausing by RNA polymerase is an underlying event in the regulation of
transcript elongation, yet the physical changes in the transcribing complex that create the …

During transcription, RNA polymerase II (RNAPII) must select the correct nucleotide,
catalyze its addition to the growing RNA transcript, and move stepwise along the DNA until a …

During transcription elongation, RNA polymerase has been assumed to attain equilibrium
between pre-and post-translocated states rapidly relative to the subsequent catalysis. Under …