What is the “modified” CTAB protocol? Characterizing modifications to the CTAB DNA extraction protocol

JJ Schenk, LE Becklund, SJ Carey… - Applications in Plant …, 2023 - Wiley Online Library
Cetyltrimethylammonium bromide (CTAB)–based methods are widely used to isolate DNA
from plant tissues, but the unique chemical composition of secondary metabolites among …

[HTML][HTML] Emerging methods in botanical DNA/RNA extraction

N Mitchell, EV McAssey, RGJ Hodel - Applications in Plant …, 2023 - ncbi.nlm.nih.gov
Analyses of nucleic acids (DNA and RNA) have become a staple tool for botanists to answer
questions across a wide variety of disciplines, ranging from population genetics to …

[HTML][HTML] Cationic and anionic detergent buffers in sequence yield high-quality genomic DNA from diverse plant species

S Krishnan, S Sasi, P Kodakkattumannil… - Analytical …, 2024 - Elsevier
Because of the heterogeneity among seedlings of outbreeding species, the use of seedling
tissues as a source of DNA is unsuitable for the genomic characterization of elite …

Genetic diversity and population structure of Polygonatum cyrtonema Hua in China using SSR markers

H Liu, H Cheng, J Xu, J Hu, C Zhao, L Xing, M Wang… - Plos one, 2023 - journals.plos.org
Polygonatum cyrtonema Hua is a perennial herbaceous plant of the Polygonatum genus,
belonging to the Liliaceae family, with significant medicinal and nutritional value. In China …

Complete Chloroplast Genomes and the Phylogenetic Analysis of Three Native Species of Paeoniaceae from the Sino-Himalayan Flora Subkingdom

H Cai, R Xu, P Tian, M Zhang, L Zhu, T Yin… - International Journal of …, 2023 - mdpi.com
Paeonia delavayi var. lutea, Paeonia delavayi var. angustiloba, and Paeonia ludlowii are
Chinese endemics that belong to the Paeoniaceae family and have vital medicinal and …

Optimizing efficient PCR‐amplifiable DNA extraction from herbarium specimens

FE Gouker, Y Guo, HT Svoboda… - Applications in Plant …, 2023 - Wiley Online Library
Premise The objective of this study was to optimize an existing DNA extraction protocol for
recalcitrant plant taxa to obtain high‐quality DNA from preserved herbarium tissue suitable …

[PDF][PDF] Comparison of Three Methods to Extract Plasmodium falciparum DNA from Whole Blood and Dried Blood Spots

T Thongseesuksai, T Boonmars… - … American Journal of …, 2024 - media.malariaworld.org
This study aimed to compare the effectiveness of three DNA extraction methods: the GF-1
Blood DNA Extraction Kit (GF-1 BD Kit), which employs a spin column along with lysing and …

A rapid and efficient DNA extraction method from high oily content seeds: Ricinus communis L.-apt for PCR based assay

ERS SOLIMAN - Acta agriculturae Slovenica, 2023 - journals.uni-lj.si
Ricinus communis seeds harbor high oil and polyphenolics contents that hinder DNA
extraction. Here, a rapid and efficient protocol for isolating total DNA from R. communis …

High-molecular-weight DNA extraction for broadleaved and conifer tree species

M Krautwurst, A Eikhof, S Winkler, D Bross, B Kersten… - Silvae Genetica - sciendo.com
Plant genome sequencing based on long reads has increasingly been applied also to tree
species in recent years. A crucial step in these genome projects is the successful extraction …