[HTML][HTML] Major advances in targeted protein degradation: PROTACs, LYTACs, and MADTACs
SB Alabi, CM Crews - Journal of Biological Chemistry, 2021 - ASBMB
Of late, targeted protein degradation (TPD) has surfaced as a novel and innovative chemical
tool and therapeutic modality. By co-opting protein degradation pathways, TPD facilitates …
tool and therapeutic modality. By co-opting protein degradation pathways, TPD facilitates …
New insights into genome folding by loop extrusion from inducible degron technologies
Chromatin folds into dynamic loops that often span hundreds of kilobases and physically
wire distant loci together for gene regulation. These loops are continuously created …
wire distant loci together for gene regulation. These loops are continuously created …
In silico protein interaction screening uncovers DONSON's role in replication initiation
CDC45-MCM2-7-GINS (CMG) helicase assembly is the central event in eukaryotic
replication initiation. In yeast, a multi-subunit “pre-loading complex”(pre-LC) accompanies …
replication initiation. In yeast, a multi-subunit “pre-loading complex”(pre-LC) accompanies …
[HTML][HTML] A mitotic chromatin phase transition prevents perforation by microtubules
Dividing eukaryotic cells package extremely long chromosomal DNA molecules into discrete
bodies to enable microtubule-mediated transport of one genome copy to each of the newly …
bodies to enable microtubule-mediated transport of one genome copy to each of the newly …
RAD51 bypasses the CMG helicase to promote replication fork reversal
Replication fork reversal safeguards genome integrity as a replication stress response. DNA
translocases and the RAD51 recombinase catalyze reversal. However, it remains unknown …
translocases and the RAD51 recombinase catalyze reversal. However, it remains unknown …
An expanded auxin-inducible degron toolkit for Caenorhabditis elegans
GE Ashley, T Duong, MT Levenson, MAQ Martinez… - Genetics, 2021 - academic.oup.com
The auxin-inducible degron (AID) system has emerged as a powerful tool to conditionally
deplete proteins in a range of organisms and cell types. Here, we describe a toolkit to …
deplete proteins in a range of organisms and cell types. Here, we describe a toolkit to …
[HTML][HTML] SARS-CoV-2 restructures host chromatin architecture
Some viruses restructure host chromatin, influencing gene expression, with implications for
disease outcome. Whether this occurs for SARS-CoV-2, the virus causing COVID-19, is …
disease outcome. Whether this occurs for SARS-CoV-2, the virus causing COVID-19, is …
[HTML][HTML] Genome-wide analysis of the interplay between chromatin-associated RNA and 3D genome organization in human cells
The interphase genome is dynamically organized in the nucleus and decorated with
chromatin-associated RNA (caRNA). It remains unclear whether the genome architecture …
chromatin-associated RNA (caRNA). It remains unclear whether the genome architecture …
[HTML][HTML] The Toxoplasma micropore mediates endocytosis for selective nutrient salvage from host cell compartments
W Wan, H Dong, DH Lai, J Yang, K He, X Tang… - Nature …, 2023 - nature.com
Apicomplexan parasite growth and replication relies on nutrient acquisition from host cells,
in which intracellular multiplication occurs, yet the mechanisms that underlie the nutrient …
in which intracellular multiplication occurs, yet the mechanisms that underlie the nutrient …
[HTML][HTML] Mitotic DNA synthesis in response to replication stress requires the sequential action of DNA polymerases zeta and delta in human cells
W Wu, SA Barwacz, R Bhowmick, K Lundgaard… - Nature …, 2023 - nature.com
Oncogene activation creates DNA replication stress (RS) in cancer cells, which can
generate under-replicated DNA regions (UDRs) that persist until cells enter mitosis. UDRs …
generate under-replicated DNA regions (UDRs) that persist until cells enter mitosis. UDRs …