Non‐invasive and time‐resolved measurement of the respiration activity of Chinese hamster ovary cells enables prediction of key culture parameters in shake flasks
Background Shake flasks are frequently used for mammalian cell suspension cultures. For
process development and routine culture monitoring, information on culture behavior is …
process development and routine culture monitoring, information on culture behavior is …
[HTML][HTML] Impact of hydromechanical stress on CHO cells' metabolism and productivity: Insights from shake flask cultivations with online monitoring of the respiration …
A Neuss, JST Borges, N von Vegesack, J Büchs… - New …, 2024 - Elsevier
The hydromechanical stress is a relevant parameter for mammalian cell cultivations,
especially regarding scale-up processes. It describes the mechanical forces exerted on cells …
especially regarding scale-up processes. It describes the mechanical forces exerted on cells …
Scale‐down of CHO cell cultivation from shake flasks based on oxygen mass transfer allows application of parallelized, non‐invasive, and time‐resolved monitoring of …
Cultivating Chinese hamster ovary (CHO) cells in microtiter plates (MTPs) with time‐
resolved monitoring of the oxygen transfer rate (OTR) is highly desirable to provide process …
resolved monitoring of the oxygen transfer rate (OTR) is highly desirable to provide process …
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations
SMW Wahjudi, T Petrzik, F Oudenne… - Biotechnology …, 2023 - Wiley Online Library
Costly complex media components such as yeast extract and peptone are still widely used
in industrial bioprocesses, despite their ill‐defined composition. Side stream products such …
in industrial bioprocesses, despite their ill‐defined composition. Side stream products such …
Introducing oxygen transfer rate measurements as a novel method for time-resolved cytotoxicity assessment in shake flasks
N Ihling, LP Munkler, R Paul, D Lang… - Environmental Sciences …, 2022 - Springer
Background Determining the cytotoxicity of test substances is essential for the safety
assessment of chemicals. To quantify the cytotoxicity, dose–response curves are determined …
assessment of chemicals. To quantify the cytotoxicity, dose–response curves are determined …
Sampling-free investigation of microbial carbon source preferences on renewable feedstocks via online monitoring of oxygen transfer rate
LA Grebe, P Richter, T Altenkirch, M Mann… - Bioprocess and …, 2024 - Springer
The transition towards sustainable bioprocesses requires renewable feedstocks to reduce
dependency on finite resources. While plant-based feedstocks offer significant potential …
dependency on finite resources. While plant-based feedstocks offer significant potential …
Online monitoring of the respiration activity in 96‐deep‐well microtiter plate Chinese hamster ovary cultures streamlines kill curve experiments
A Neuss, N von Vegesack, R Liepelt… - Biotechnology …, 2024 - Wiley Online Library
Cell line generation of mammalian cells is a time‐consuming and labor‐intensive process,
especially because of challenges in clone selection after transfection. Antibiotics are …
especially because of challenges in clone selection after transfection. Antibiotics are …
Experimental kS estimation: A comparison of methods for Corynebacterium glutamicum from lab to microfluidic scale
H Steinhoff, M Finger, M Osthege… - Biotechnology and …, 2023 - Wiley Online Library
Abstract Knowledge about the specific affinity of whole cells toward a substrate, commonly
referred to as kS, is a crucial parameter for characterizing growth within bioreactors. State‐of …
referred to as kS, is a crucial parameter for characterizing growth within bioreactors. State‐of …
Development of an itaconic acid production process on alternative feedstocks
Kurzfassung Die wirtschaftlichen Nachteile von Bioraffineriekonzepten, im Vergleich zu
konventionellen petrochemischen Produktionswegen, behindern deren Etablierung in einer …
konventionellen petrochemischen Produktionswegen, behindern deren Etablierung in einer …
Filamentous co-cultivation for the conversion of cellulose into pigmented antibiotics
M Finger, J Büchs, M Agler-Rosenbaum - 2023 - publications.rwth-aachen.de
Kurzfassung Der Bedarf an Naturprodukten wie Antibiotika ist größer denn je. Angetrieben
durch aufkommende Antibiotikaresistenzen sind neue Produktionsverfahren von …
durch aufkommende Antibiotikaresistenzen sind neue Produktionsverfahren von …