Recent progress in quantitative phosphoproteomics

K Zittlau, P Nashier, C Cavarischia-Rega… - Expert Review of …, 2023 - Taylor & Francis
Introduction Protein phosphorylation is a critical post-translational modification involved in
the regulation of numerous cellular processes from signal transduction to modulation of …

Type III-B CRISPR-Cas cascade of proteolytic cleavages

JA Steens, JPK Bravo, CRP Salazar, C Yildiz… - Science, 2024 - science.org
The generation of cyclic oligoadenylates and subsequent allosteric activation of proteins that
carry sensory domains is a distinctive feature of type III CRISPR-Cas systems. In this work …

More than two components: complexities in bacterial phosphosignaling

A Frando, C Grundner - Msystems, 2024 - Am Soc Microbiol
For over 40 years, the two-component systems (TCSs) have taken front and center in our
thinking about the signaling mechanisms by which bacteria sense and respond to their …

Deep phosphoproteomics of Klebsiella pneumoniae reveals HipA-mediated tolerance to ciprofloxacin

P Nashier, I Samp, M Adler, F Ebner, LT Lê… - PLoS …, 2024 - journals.plos.org
Klebsiella pneumoniae belongs to the group of bacterial pathogens causing the majority of
antibiotic-resistant nosocomial infections worldwide; however, the molecular mechanisms …

Cyclic di-GMP as an antitoxin regulates bacterial genome stability and antibiotic persistence in biofilms

H Liao, X Yan, C Wang, C Huang, W Zhang, L Xiao… - Elife, 2024 - elifesciences.org
Biofilms are complex bacterial communities characterized by a high persister prevalence,
which contributes to chronic and relapsing infections. Historically, persister formation in …

Reverse-Phase Protein Microarrays for Overexpressed Escherichia coli Lysates Reveal a Novel Tyrosine Kinase

BB Keskin, CS Chen, PS Tsai, PX Du… - Analytical …, 2024 - ACS Publications
Tyrosine phosphorylation is one of the most important posttranslational modifications in
bacteria, linked to regulating growth, migration, virulence, secondary metabolites, biofilm …

A type II toxin–antitoxin system, ECs3274-ECs3275, in enterohemorrhagic Escherichia coli O157

Y Sasaki, Y Mogi, M Yoshioka, K Liu… - Bioscience …, 2025 - academic.oup.com
The toxin–antitoxin (TA) genetic module controls various bacterial events. Novel toxins with
different functions are still being discovered. This study aimed to determine whether the …

Advancements in Global Phosphoproteomics Profiling: Overcoming Challenges in Sensitivity and Quantification

G Muneer, CS Chen, YJ Chen - Proteomics, 2024 - Wiley Online Library
Protein phosphorylation introduces post‐genomic diversity to proteins, which plays a crucial
role in various cellular activities. Elucidation of system‐wide signaling cascades requires …

Indole inhibited the expression of csrA gene in Escherichia coli

J Zheng, G Zuo, Z Zhou, Z Shi, H Guo… - The Journal of General …, 2023 - jstage.jst.go.jp
抄録 Indole is a very important signal molecule which plays multiple regulatory roles in many
physiological and biochemical processes of bacteria, but up to now, the reasons for its wide …

An Advanced Bacterial Single-cell RNA-seq Reveals Biofilm Heterogeneity

Y Pu, X Yan, H Liao, C Wang, C Huang, W Zhang… - 2024 - researchsquare.com
In contrast to mammalian cells, bacterial cells lack mRNA polyadenylated tails, presenting a
hurdle in isolating mRNA amidst the prevalent rRNA during single-cell RNA-seq. This study …