Current advances of Pichia pastoris as cell factories for production of recombinant proteins

Y Pan, J Yang, J Wu, L Yang, H Fang - Frontiers in Microbiology, 2022 - frontiersin.org
Pichia pastoris (syn. Komagataella spp.) has attracted extensive attention as an efficient
platform for recombinant protein (RP) production. For obtaining a higher protein titer, many …

[HTML][HTML] Komagataella phaffii (Pichia pastoris) as a Powerful Yeast Expression System for Biologics Production

Y Unver, I Dagci - Frontiers in Bioscience-Elite, 2024 - imrpress.com
Komagataella phaffii (K. phaffii)(Pichia pastoris), also called biotech yeast, is a yeast species
with many applications in the biotechnology and pharmaceutical industries. This …

Affinity-based and in a single step purification of recombinant horseradish peroxidase A2A isoenzyme produced by Pichia pastoris

M Acar, N Abul, S Yildiz, ED Taskesenligil… - Bioprocess and …, 2023 - Springer
Horseradish peroxidase (HRP) is an oxidoreductase enzyme and oxidizes various inorganic
and organic compounds. It has wide application areas such as immunological tests, probe …

Design and heterologous expression of a novel dimeric LL37 variant in Pichia pastoris

N Zhan, L Zhang, H Yang, Y Zheng, X Wei… - Microbial Cell …, 2021 - Springer
Background The antimicrobial peptide LL37 is produced by white blood cells (mainly
neutrophils) and various epithelial cells, and has the outstanding advantages of participating …

Heterologous expression of azurin from Pseudomonas aeruginosa in the yeast Pichia pastoris

Y Unver, B Sensoy Gun, M Acar… - Preparative Biochemistry …, 2021 - Taylor & Francis
Azurin, which is a bacterial secondary metabolite has been attracted as a potential
anticancer agent in recent years because induced death of cancer cells and inhibited their …

Basal transcription profiles of the rhamnose-inducible promoter PLRA3 and the development of efficient PLRA3-based systems for markerless gene deletion and a mutant …

J Jiao, S Wang, M Liang, Y Zhang, X Xu, W Zhang… - Current genetics, 2019 - Springer
An ideal inducible promoter presents inducibility with an inducer and no basal transcription
without inducer. Previous studies have shown that P LRA3 in Pichia pastoris is a strong …

Constitutive and extracellular expression of pectin methylesterase from Pectobacterium chrysanthemi in Pichia pastoris

M Acar, Y Unver - 3 Biotech, 2022 - Springer
Pectin methylesterase (PME) which is widely used in the cosmetic, food and pharmaceutical
industries catalyses the hydrolysis of the methyl ester of pectin to yield methanol and free …

Azurin proteininin Pichia pastoris ile rekombinant üretimi

B Şensoy Gün - acikbilim.yok.gov.tr
Bu çalışmada Pichia pastoris ekspresyon sisteminde sıklıkla kullanılan ve metanolle
indüklenebilen AOX (alkol oksidaz) promotörünün kontrolü altında azurin proteininin üretimi …

Pectobacterium chrysanthemi pektin metilesteraz enziminin Pichia pastoris ile rekombinant üretimi

M Acar - acikbilim.yok.gov.tr
Bitkilerin hücre duvarının en önemli bileşeni olan, tüm canlı bitkilerin hücre duvarında ve
hücreleri arasında bulunan pektini degrade eden pektinazlar, gıda enzimlerinin küresel …

[引用][C] Manyetik nanopartikül aracılığıyla Pichia pastorise yeni bir gen aktarım yönteminin geliştirilmesi

Ş Yildiz - Fen Bilimleri Enstitüsü