Global environmental issues and sustainable development call for new technologies for fine
chemical synthesis and waste valorization. Biocatalysis has attracted great attention as the …

Directed evolution has emerged as a powerful strategy to engineer various properties of
proteins. Traditional methods to construct libraries such as error-prone PCR and DNA …

In recent years, the engineering of flexible loops to improve enzyme properties has gained
attention in biocatalysis. Herein, we report a loop engineering strategy to improve the …

Esterification is one of the most important reactions in organic synthesis and industrial
applications. The applications of lipases in esterification are increasingly widespread and …

Owing to the environmental friendliness and vast advantages that enzymes offer in the
biotechnology and industry fields, biocatalysts are a prolific investigation field. However, the …

Cytochrome P450s are heme-thiolate enzymes that have been broadly applied in
pharmaceutical and biosynthesis because of their efficient oxidation at inert carbons …

Bacillus proteases commonly exhibit remarkably reduced activity under cold conditions.
Herein, we employed a tailored combination of a loop engineering strategy and iterative …

The narrow substrate scope limits the wide industrial application of enzymes. Here, we
successfully broadened the substrate scope of a nitrile hydratase (NHase) through mutation …

Understanding structure–function relationships in proteins is pivotal in their development as
industrial biocatalysts. In this regard, rational engineering of protein active site access …

Fatty acid photodecarboxylase (FAP) is one of the few photoenzymes in nature. The ability of
FAP to convert fatty acids into alka (e) nes without the need for reducing equivalents put this …