Escherichia coli acetyl-CoA carboxylase (ACC), the enzyme responsible for synthesis of
malonyl-CoA, the building block of fatty acid synthesis, is the paradigm bacterial ACC. Many …

Abstract Data-Independent Acquisition (DIA) is a method to improve consistent identification
and precise quantitation of peptides and proteins by mass spectrometry (MS). The targeted …

Type II toxin–antitoxin (TA) systems are widely distributed in bacterial and archaeal
genomes and are involved in diverse critical cellular functions such as defense against …

E. coli is one of the most widely used organisms for understanding the principles of cellular
and molecular genetics. However, we are yet to understand the origin of several …

Repurposing natural systems to develop customized functions in biological systems is one
of the main thrusts of synthetic biology. Translational coupling is a common phenomenon in …

During proteomics data analysis, experimental spectra are searched against a user-defined
protein database consisting of proteins that are reasonably expected to be present in the …

Bacterial second messenger signaling often plays an important role in cellular physiology. In
this study, we have attempted to understand how c-di-AMP synthesis and degradation are …

Amino acid ligation to cognate transfer RNAs (tRNAs) is catalyzed by aminoacyl-tRNA
synthetases (aaRSs)—essential interpreters of the genetic code during translation …

To swim up gradients of nutrients, E. coli senses nutrient concentrations within its periplasm.
For small nutrient molecules, periplasmic concentrations typically match extracellular …

Bacterial second messenger signaling often plays an important role in cellular physiology. In
this study, we have attempted to understand how c-di-AMP synthesis and degradation are …